MetaCyc Pathway: superpathway of heme b biosynthesis from glutamate in Pseudomonas fluorescens FW300-N2E2

Reactions and Genes	Rifampicin 0.003 mg/ml
Glutamate--tRNA ligase: L-glutamate + ATP + a tRNAglu→an L-glutamyl-[tRNAGlu] + AMP + diphosphate (EC 6.1.1.17)
Pf6N2E2_53	N.D.
Glutamyl-tRNA reductase (in reverse): an L-glutamyl-[tRNAGlu] + NADPH + H+→(S)-4-amino-5-oxopentanoate + a tRNAglu + NADP+ (EC 1.2.1.70)
Pf6N2E2_5220	N.D.
Glutamate-1-semialdehyde 2,1-aminomutase: (S)-4-amino-5-oxopentanoate→5-aminolevulinate (EC 5.4.3.8)
Pf6N2E2_1739	-0.1
Pf6N2E2_5022	N.D.
Porphobilinogen synthase: 2 5-aminolevulinate→porphobilinogen + H+ + 2 H2O (EC 4.2.1.24)
Pf6N2E2_2146	-0.3
Pf6N2E2_4452	-0.5
Hydroxymethylbilane synthase: 4 porphobilinogen + H2O→preuroporphyrinogen + 4 ammonium (EC 2.5.1.61)
Pf6N2E2_4431	N.D.
Uroporphyrinogen-III synthase: preuroporphyrinogen→uroporphyrinogen-III + H2O (EC 4.2.1.75)
Pf6N2E2_4432	+1.4
Uroporphyrinogen decarboxylase: uroporphyrinogen-III + 4 H+→coproporphyrinogen III + 4 CO2 (EC 4.1.1.37)
Pf6N2E2_3750	N.D.
Coproporphyrinogen oxidase: coproporphyrinogen III + dioxygen + 2 H+→protoporphyrinogen IX + 2 CO2 + 2 H2O (EC 1.3.3.3)
Pf6N2E2_4129	N.D.
Protoporphyrinogen oxidase: protoporphyrinogen IX + 3 dioxygen→protoporphyrin IX + 3 hydrogen peroxide (EC 1.3.3.4)
Pf6N2E2_1875	-0.1
protoporphyrin IX + Fe2+→protoheme + 2 H+ (EC 4.98.1.1)
Pf6N2E2_5233	N.D.

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information