MetaCyc Pathway: L-methionine salvage cycle III in Pseudomonas fluorescens FW300-N2E3

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Reactions and Genes perchlorate 75 mM
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S-methyl-5'-thioadenosine phosphorylase:
S-methyl-5'-thioadenosine + phosphate→S-methyl-5-thio-α-D-ribose 1-phosphate + adenine (EC 2.4.2.28)
AO353_00450 -0.1
AO353_14225 -1.3
S-methyl-5-thioribose-1-phosphate isomerase:
S-methyl-5-thio-α-D-ribose 1-phosphate→S-methyl-5-thio-D-ribulose 1-phosphate (EC 5.3.1.23)
AO353_02085 +0.4
Methylthioribulose 1-phosphate dehydratase:
S-methyl-5-thio-D-ribulose 1-phosphate→5-(methylsulfanyl)-2,3-dioxopentyl 1-phosphate + H2O (EC 4.2.1.109)
AO353_02240 +0.1
Acireductone synthase:
5-(methylsulfanyl)-2,3-dioxopentyl 1-phosphate→2-hydroxy-5-(methylsulfanyl)-3-oxopent-1-enyl 1-phosphate + H+ (EC 3.1.3.77; 5.3.2.5)
AO353_02230 N.D.
Acireductone synthase:
2-hydroxy-5-(methylsulfanyl)-3-oxopent-1-enyl 1-phosphate + H2O→1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate (EC 3.1.3.77; 3.1.3.87)
AO353_02230 N.D.
Acireductone dioxygenase (Fe(2+)-requiring):
1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + dioxygen→4-(methylsulfanyl)-2-oxobutanoate + formate + H+ (EC 1.13.11.54)
AO353_02235 -0.4
4-(methylsulfanyl)-2-oxobutanoate + L-glutamine→2-oxoglutaramate + L-methionine (EC 2.6.1.117)
No genes
2-oxoglutaramate + H2O→2-oxoglutarate + ammonium (EC 3.5.1.111; 3.5.1.3)
AO353_15920 +0.2
Methionine adenosyltransferase:
L-methionine + ATP + H2O→S-adenosyl-L-methionine + diphosphate + phosphate (EC 2.5.1.6)
AO353_07965 N.D.
Adenosylmethionine decarboxylase:
S-adenosyl-L-methionine + H+→S-adenosyl 3-(methylsulfanyl)propylamine + CO2 (EC 4.1.1.50)
AO353_07190 -0.0
Spermidine synthase:
S-adenosyl 3-(methylsulfanyl)propylamine + putrescine→spermidine + S-methyl-5'-thioadenosine + H+ (EC 2.5.1.16)
AO353_02195 -0.0
AO353_19120 +0.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information