MetaCyc Pathway: peptidoglycan recycling II in Pseudomonas fluorescens FW300-N1B4

Reactions and Genes	Choline chloride 200 mM
a mature peptidoglycan (meso-DAP containing)→a mature peptidoglycan (meso-DAP containing) + GlcNAc-1,6-anhMurNAc-L-Ala-γ-D-Glu-meso-DAP-D-Ala (EC 4.2.2.f)
Pf1N1B4_2694	+1.5
GlcNAc-1,6-anhMurNAc-L-Ala-γ-D-Glu-meso-DAP-D-Ala[out] + H+[out]→GlcNAc-1,6-anhMurNAc-L-Ala-γ-D-Glu-meso-DAP-D-Ala[in] + H+[in]
No genes
N-acetylmuramoyl-L-alanine amidase: GlcNAc-1,6-anhMurNAc-L-Ala-γ-D-Glu-meso-DAP-D-Ala + H2O→N-acetyl-β-D-glucosamine-1,6-anhydro-N-acetyl-β-D-muramate + L-alanyl-γ-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanine (EC 3.5.1.28)
Pf1N1B4_1151	N.D.
Pf1N1B4_1415	N.D.
Pf1N1B4_1882	+0.4
Pf1N1B4_1991	-0.2
Beta-N-acetylhexosaminidase: N-acetyl-β-D-glucosamine-1,6-anhydro-N-acetyl-β-D-muramate + H2O→1,6-anhydro-N-acetyl-β-D-muramate + N-acetyl-D-glucosamine (EC 3.2.1.52)
Pf1N1B4_3912	N.D.
Muramoyltetrapeptide carboxypeptidase: L-alanyl-γ-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanine + H2O→L-alanyl-γ-D-glutamyl-meso-diaminopimelate + D-alanine (EC 3.4.17.13)
Pf1N1B4_2700	N.D.
1,6-anhydro-N-acetyl-β-D-muramate + ATP + H2O→N-acetyl-D-muramate 6-phosphate + ADP + H+ (EC 2.7.1.170)
Pf1N1B4_2562	N.D.
N-acetyl-D-muramate 6-phosphate + H2O→N-acetyl-D-muramate + phosphate (EC 3.1.3.105)
Pf1N1B4_357	N.D.
N-acetyl-D-muramate + ATP→N-acetyl-α-D-muramate 1-phosphate + ADP + H+ (EC 2.7.1.221)
Pf1N1B4_2532	N.D.
N-acetyl-α-D-muramate 1-phosphate + H+ + UDP→UDP-N-acetyl-α-D-muramate + phosphate (EC 2.7.7.99)
Pf1N1B4_2533	N.D.
L-alanyl-γ-D-glutamyl-meso-diaminopimelate + UDP-N-acetyl-α-D-muramate + ATP→UDP-MurNAc-L-Ala-γ-D-Glu-meso-DAP + ADP + H+ + phosphate (EC 6.3.2.45)
Pf1N1B4_2676	N.D.

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information