MetaCyc Pathway: TCA cycle VI (Helicobacter) in Pseudomonas fluorescens FW300-N1B4

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Reactions and Genes methylglyoxal 0.008 vol%
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2-oxoglutarate synthase:
2-oxoglutarate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→succinyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.3)
No genes
3-oxoacid CoA-transferase:
succinyl-CoA + acetoacetate→succinate + acetoacetyl-CoA (EC 2.8.3.5)
Pf1N1B4_5833 +0.5
Pf1N1B4_5834 +0.1
Succinate dehydrogenase (ubiquinone) (in reverse):
succinate[in] + a menaquinone→fumarate[in] + a menaquinol (EC 1.3.5.1)
No genes
Fumarate hydratase (in reverse):
fumarate + H2O→(S)-malate (EC 4.2.1.2)
Pf1N1B4_1088 N.D.
Pf1N1B4_367 +1.2
Pf1N1B4_683 +1.0
(S)-malate + a menaquinone→oxaloacetate + a menaquinol (EC 1.1.5.4)
Pf1N1B4_2939 +0.4
Pf1N1B4_4189 +0.3
Citrate (Si)-synthase:
oxaloacetate + acetyl-CoA + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
Pf1N1B4_3676 N.D.
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
Pf1N1B4_3821 +0.5
Pf1N1B4_3888 +0.1
Pf1N1B4_4564 N.D.
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
Pf1N1B4_3821 +0.5
Pf1N1B4_3888 +0.1
Pf1N1B4_4564 N.D.
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→2-oxoglutarate + CO2 + NADPH (EC 1.1.1.42)
Pf1N1B4_4050 N.D.
Pf1N1B4_4051 -0.5
Pf1N1B4_4052 -0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information