MetaCyc Pathway: superpathway of L-methionine biosynthesis (by sulfhydrylation) in Pseudomonas fluorescens FW300-N1B4

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Reactions and Genes Doxycycline hyclate 0.0001 mg/ml
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
Pf1N1B4_160 -0.0
Pf1N1B4_2018 +0.0
Pf1N1B4_2139 -0.2
Pf1N1B4_261 +0.1
Pf1N1B4_3564 -0.4
Pf1N1B4_3771 +0.0
Pf1N1B4_386 -0.3
Pf1N1B4_4186 +0.1
Pf1N1B4_4425 +0.2
Pf1N1B4_4646 +0.8
Pf1N1B4_4672 N.D.
Pf1N1B4_4996 -0.1
Pf1N1B4_5070 +0.2
Pf1N1B4_510 +0.2
Pf1N1B4_5999 -0.4
Pf1N1B4_818 -0.2
Pf1N1B4_849 +0.2
Sulfate adenylyltransferase:
sulfate + ATP + H+→adenosine 5'-phosphosulfate + diphosphate (EC 2.7.7.4)
Pf1N1B4_1057 -2.5
Pf1N1B4_1058 N.D.
Adenylyl-sulfate kinase:
adenosine 5'-phosphosulfate + ATP→3'-phosphoadenylyl-sulfate + ADP + H+ (EC 2.7.1.25)
Pf1N1B4_1057 -2.5
Pf1N1B4_1757 N.D.
Pf1N1B4_334 N.D.
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
Pf1N1B4_3451 +0.4
Phosphoadenylyl-sulfate reductase (thioredoxin) (in reverse):
3'-phosphoadenylyl-sulfate + a reduced thioredoxin→sulfite + adenosine 3',5'-bisphosphate + an oxidized thioredoxin + 2 H+ (EC 1.8.4.8)
Pf1N1B4_3812 -0.1
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
Pf1N1B4_10 -0.3
Pf1N1B4_11 N.D.
Pf1N1B4_3225 N.D.
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
Pf1N1B4_3013 N.D.
Sulfite reductase (NADPH) (in reverse):
sulfite + 3 NADPH + 5 H+→hydrogen sulfide + 3 NADP+ + 3 H2O (EC 1.8.1.2)
Pf1N1B4_1142 -2.1
Pf1N1B4_1814 +0.3
Pf1N1B4_4656 -0.3
Pf1N1B4_5665 -0.3
Homoserine O-acetyltransferase:
L-homoserine + acetyl-CoA→O-acetyl-L-homoserine + coenzyme A (EC 2.3.1.31)
Pf1N1B4_2337 +0.1
Pf1N1B4_2338 +0.1
O-acetylhomoserine aminocarboxypropyltransferase:
O-acetyl-L-homoserine + hydrogen sulfide→L-homocysteine + acetate + H+ (EC 2.5.1.49)
Pf1N1B4_3963 -0.8
Pf1N1B4_4893 -0.2
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase:
L-homocysteine + 5-methyltetrahydropteroyl tri-L-glutamate→L-methionine + tetrahydropteroyl tri-L-glutamate (EC 2.1.1.14)
Pf1N1B4_783 +0.3
Methionine synthase:
L-homocysteine + a 5-methyltetrahydrofolate→L-methionine + a tetrahydrofolate (EC 2.1.1.13)
Pf1N1B4_5661 -0.7

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information