MetaCyc Pathway: L-valine degradation I in Pseudomonas stutzeri RCH2

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Reactions and Genes nitrate 250 mM
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Branched-chain-amino-acid transaminase:
L-valine + 2-oxoglutarate→3-methyl-2-oxobutanoate + L-glutamate (EC 2.6.1.42)
Psest_0450 -0.4
2-oxoisovalerate dehydrogenase (acylating):
3-methyl-2-oxobutanoate + coenzyme A + NAD+→isobutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
isobutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→methylacrylyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
Psest_2440 +0.5
Psest_2445 +0.4
methylacrylyl-CoA + H2O→(S)-3-hydroxy-isobutanoyl-CoA (EC 4.2.1.150)
Psest_2076 +0.3
Psest_2437 -0.1
Psest_2654 N.D.
Psest_3109 +0.2
Psest_3969 -1.3
Psest_4235 +0.6
3-hydroxyisobutyryl-CoA hydrolase:
(S)-3-hydroxy-isobutanoyl-CoA + H2O→(S)-3-hydroxy-isobutanoate + coenzyme A + H+ (EC 3.1.2.4)
Psest_2439 +0.1
Psest_3110 -0.4
3-hydroxyisobutyrate dehydrogenase:
(S)-3-hydroxy-isobutanoate + NAD+→(S)-methylmalonate-semialdehyde + NADH + H+ (EC 1.1.1.31)
Psest_1322 -0.5
Psest_2438 -0.1
Psest_2654 N.D.
Psest_3800 +0.0
Psest_3969 -1.3
Psest_4235 +0.6
(S)-3-amino-2-methylpropionate transaminase (in reverse):
(S)-methylmalonate-semialdehyde + L-glutamate→(S)-3-amino-2-methylpropanoate + 2-oxoglutarate (EC 2.6.1.22)
No genes
Methylmalonate-semialdehyde dehydrogenase (acylating):
(S)-methylmalonate-semialdehyde + coenzyme A + NAD+ + H2O→propanoyl-CoA + hydrogen carbonate + NADH + H+ (EC 1.2.1.27)
Psest_2436 -0.2
Psest_3781 -0.3

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information