MetaCyc Pathway: fatty acid β-oxidation VI (mammalian peroxisome) in Pseudomonas stutzeri RCH2

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Reactions and Genes nitrate 250 mM
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a 2,3,4-saturated fatty acid + ATP + coenzyme A→a 2,3,4-saturated fatty acyl CoA + AMP + diphosphate
No genes
Acyl-CoA oxidase:
a 2,3,4-saturated fatty acyl CoA + dioxygen→a (2E)-2-enoyl-CoA + hydrogen peroxide
(EC 1.3.3.6)
No genes
Enoyl-CoA hydratase (in reverse):
a (2E)-2-enoyl-CoA + H2O→a 2,3,4-saturated (3S)-3-hydroxyacyl-CoA
(EC 4.2.1.17)
Psest_2076 +0.3
Psest_2437 -0.1
Psest_2439 +0.1
Psest_2654 N.D.
Psest_2720 +0.3
Psest_3109 +0.2
Psest_3110 -0.4
Psest_3800 +0.0
Psest_3969 -1.3
Psest_4235 +0.6
a (2E)-2-enoyl-CoA + H2O→a 2,3,4-saturated (3R)-3-hydroxyacyl-CoA (EC 4.2.1.119)
Psest_1333 N.D.
3-hydroxyacyl-CoA dehydrogenase:
a 2,3,4-saturated (3S)-3-hydroxyacyl-CoA + NAD+→a 2,3,4-saturated 3-oxoacyl-CoA + NADH + H+
(EC 1.1.1.35)
Psest_2654 N.D.
Psest_3800 +0.0
Psest_3969 -1.3
Psest_4235 +0.6
a 2,3,4-saturated (3R)-3-hydroxyacyl-CoA + NAD+→a 2,3,4-saturated 3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.M19)
No genes
Acetyl-CoA C-acyltransferase (in reverse):
a 2,3,4-saturated 3-oxoacyl-CoA + coenzyme A→a 2,3,4-saturated fatty acyl CoA + acetyl-CoA
(EC 2.3.1.16)
Psest_1076 +0.2
Psest_1829 +0.6
Psest_2446 -0.7
Psest_2476 -1.0
Psest_2653 N.D.
Psest_3070 -3.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information