MetaCyc Pathway: cis-vaccenate biosynthesis in Burkholderia phytofirmans PsJN

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Reactions and Genes 36hr PHYTO10 MOI 10
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Beta-ketoacyl-acyl-carrier-protein synthase II:
a palmitoleoyl-[acp] + a malonyl-[acp] + H+→an (11Z)-3-oxooctadec-11-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
BPHYT_RS14415 N.D.
BPHYT_RS15130 +1.2
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
an (11Z)-3-oxooctadec-11-enoyl-[acp] + NADPH + H+→a (3R,11Z)-3-hydroxyoctadec-11-enoyl-[acp] + NADP+ (EC 1.1.1.100)
BPHYT_RS01050 +0.4
BPHYT_RS04770 -0.0
BPHYT_RS05365 +0.5
BPHYT_RS05385 +0.2
BPHYT_RS06420 N.D.
BPHYT_RS07065 +0.2
BPHYT_RS08285 N.D.
BPHYT_RS08365 +0.0
BPHYT_RS08890 +0.0
BPHYT_RS10170 +0.3
BPHYT_RS10200 N.D.
BPHYT_RS10595 -0.3
BPHYT_RS10630 -1.3
BPHYT_RS10735 N.D.
BPHYT_RS11845 N.D.
BPHYT_RS13055 -0.5
BPHYT_RS14425 N.D.
BPHYT_RS15135 +1.2
BPHYT_RS15890 -0.1
BPHYT_RS16040 N.D.
BPHYT_RS16940 -0.6
BPHYT_RS20510 N.D.
BPHYT_RS20625 +0.0
BPHYT_RS20775 -1.3
BPHYT_RS21600 -0.2
BPHYT_RS22135 -0.6
BPHYT_RS25030 -0.1
BPHYT_RS25415 N.D.
BPHYT_RS25655 -0.4
BPHYT_RS26470 -2.7
BPHYT_RS28250 N.D.
BPHYT_RS30440 +0.4
BPHYT_RS30465 -1.4
BPHYT_RS30795 +0.1
BPHYT_RS31385 +0.5
BPHYT_RS31405 N.D.
BPHYT_RS31610 -0.1
BPHYT_RS31900 -0.9
BPHYT_RS33140 N.D.
BPHYT_RS33705 -0.1
BPHYT_RS34945 N.D.
BPHYT_RS34965 N.D.
BPHYT_RS35505 -0.8
BPHYT_RS35675 -2.4
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,11Z)-3-hydroxyoctadec-11-enoyl-[acp]→a (2E,11Z)-octadeca-2,11-dienoyl-[acp] + H2O (EC 4.2.1.59)
BPHYT_RS12085 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,11Z)-octadeca-2,11-dienoyl-[acp] + NADH + H+→a cis-vaccenoyl-[acp] + NAD+ (EC 1.3.1.9)
BPHYT_RS01880 +0.0
BPHYT_RS12390 +0.1
BPHYT_RS20615 +0.2
BPHYT_RS30015 N.D.
BPHYT_RS33380 +0.3
Oleoyl-[acyl-carrier-protein] hydrolase:
a cis-vaccenoyl-[acp] + H2O→cis-vaccenate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
BPHYT_RS09490 -0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information