MetaCyc Pathway: L-glutamate and L-glutamine biosynthesis in Burkholderia phytofirmans PsJN

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Reactions and Genes Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
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Glutamate dehydrogenase (NAD(P)(+)) (in reverse):
2-oxoglutarate + ammonium + NAD(P)H + H+→L-glutamate + NAD(P)+ + H2O
(EC 1.4.1.3)
BPHYT_RS16705 N.D.
Glutamate synthase (ferredoxin) (in reverse):
2-oxoglutarate + L-glutamine + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→2 L-glutamate + 2 an oxidized ferredoxin [iron-sulfur] cluster
(EC 1.4.7.1)
BPHYT_RS03355 +0.2
Glutamate synthase (NADH) (in reverse):
2-oxoglutarate + L-glutamine + NADH + H+→2 L-glutamate + NAD+
(EC 1.4.1.14)
BPHYT_RS13315 +0.6
BPHYT_RS17850 N.D.
Glutamate synthase (NADPH):
L-glutamine + H2O→L-glutamate + ammonia + H+
(EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
BPHYT_RS00200 +0.1
BPHYT_RS00680 +0.2
BPHYT_RS00720 -0.1
BPHYT_RS01550 N.D.
BPHYT_RS01555 N.D.
BPHYT_RS01560 N.D.
BPHYT_RS01870 +0.1
BPHYT_RS03355 +0.2
BPHYT_RS03600 +0.1
BPHYT_RS12010 N.D.
BPHYT_RS12685 N.D.
BPHYT_RS13315 +0.6
BPHYT_RS14190 -2.1
BPHYT_RS14195 -0.8
BPHYT_RS15045 -0.8
BPHYT_RS15070 +0.0
BPHYT_RS17390 -2.9
BPHYT_RS17395 -3.5
BPHYT_RS17850 N.D.
BPHYT_RS17855 -4.1
BPHYT_RS19555 +2.2
BPHYT_RS21005 +0.3
BPHYT_RS22875 +0.0
BPHYT_RS25485 N.D.
BPHYT_RS27475 N.D.
BPHYT_RS28525 +0.5
Glutamate--ammonia ligase:
L-glutamate + ammonium + ATP→L-glutamine + ADP + H+ + phosphate
(EC 6.3.1.2)
BPHYT_RS09955 +0.3
BPHYT_RS12945 N.D.
BPHYT_RS23160 +0.4
Glutamate dehydrogenase (NADP(+)) (in reverse):
2-oxoglutarate + ammonium + NADPH + H+→L-glutamate + NADP+ + H2O
(EC 1.4.1.4)
BPHYT_RS16705 N.D.
Glutamate synthase (NADPH) (in reverse):
2-oxoglutarate + ammonia + NADPH + 2 H+→L-glutamate + NADP+ + H2O
(EC 1.4.1.13; 1.4.1.14)
BPHYT_RS13315 +0.6
BPHYT_RS17850 N.D.
BPHYT_RS17855 -4.1
BPHYT_RS22875 +0.0
BPHYT_RS25485 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information