MetaCyc Pathway: NAD de novo biosynthesis II (from tryptophan) in Burkholderia phytofirmans PsJN

Reactions and Genes	Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days
Tryptophan 2,3-dioxygenase: L-tryptophan + dioxygen→N-formyl-L-kynurenine (EC 1.13.11.11; 1.13.11.52)
BPHYT_RS16035	N.D.
Arylformamidase: N-formyl-L-kynurenine + H2O→L-kynurenine + formate + H+ (EC 3.5.1.9)
BPHYT_RS09685	-0.2
BPHYT_RS16025	+0.5
Kynurenine 3-monooxygenase: L-kynurenine + NADPH + dioxygen + H+→3-hydroxy-L-kynurenine + NADP+ + H2O (EC 1.14.13.9)
No genes
Kynureninase: 3-hydroxy-L-kynurenine + H2O→3-hydroxyanthranilate + L-alanine + H+ (EC 3.7.1.3)
BPHYT_RS07275	+0.1
BPHYT_RS16030	-0.5
3-hydroxyanthranilate 3,4-dioxygenase: 3-hydroxyanthranilate + dioxygen→2-amino-3-carboxymuconate-6-semialdehyde (EC 1.13.11.6)
BPHYT_RS07265	+1.8
2-amino-3-carboxymuconate-6-semialdehyde→quinolinate + H+ + H2O (spontaneous)
BPHYT_RS07265	+1.8
Nicotinate-nucleotide diphosphorylase (carboxylating) (in reverse): quinolinate + 2 H+ + 5-phospho-α-D-ribose 1-diphosphate→β-nicotinate D-ribonucleotide + CO2 + diphosphate (EC 2.4.2.19)
BPHYT_RS15645	+0.2
Nicotinate-nucleotide adenylyltransferase: β-nicotinate D-ribonucleotide + ATP + H+→nicotinate adenine dinucleotide + diphosphate (EC 2.7.7.18)
BPHYT_RS06395	N.D.
NAD(+) synthase (glutamine-hydrolyzing): nicotinate adenine dinucleotide + ATP + L-glutamine + H2O→NAD+ + AMP + L-glutamate + diphosphate + H+ (EC 6.3.5.1)
BPHYT_RS05480	+0.1

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information