MetaCyc Pathway: (5Z)-dodecenoate biosynthesis I in Enterobacter sp. TBS_079

Add experiment(s):


Reactions and Genes Plant=SorghumBicolor_Rtx430; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB_Kan50; Time=28_days; NumberPlantsPooled=4
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3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxydecanoyl-[acp]→a (2E)-dec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
MPMX20_00864 N.D.
MPMX20_01604 N.D.
Trans-2-decenoyl-[acyl-carrier-protein] isomerase:
a (2E)-dec-2-enoyl-[acp]→a (3Z)-dec-3-enoyl-[acp] (EC 5.3.3.14)
MPMX20_01604 N.D.
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (3Z)-dec-3-enoyl-[acp] + a malonyl-[acp] + H+→a (5Z)-3-oxododec-5-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
MPMX20_01775 -0.5
MPMX20_02611 -0.1
MPMX20_03258 N.D.
MPMX20_04369 +0.1
MPMX20_04372 +0.0
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (5Z)-3-oxododec-5-enoyl-[acp] + NADPH + H+→a (3R,5Z)-3-hydroxydodec-5-enoyl-[acp] + NADP+ (EC 1.1.1.100)
MPMX20_00939 +0.1
MPMX20_01172 -0.3
MPMX20_01177 +0.1
MPMX20_01195 +0.0
MPMX20_01198 -0.2
MPMX20_01773 N.D.
MPMX20_02125 -1.2
MPMX20_02218 +0.0
MPMX20_02361 +0.2
MPMX20_02544 -1.3
MPMX20_02560 -0.1
MPMX20_03106 -0.1
MPMX20_03536 -0.1
MPMX20_04371 +0.4
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,5Z)-3-hydroxydodec-5-enoyl-[acp]→a (2E,5Z)-dodeca-3,5-dienoyl-[acp] + H2O (EC 4.2.1.59)
MPMX20_00864 N.D.
MPMX20_01604 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,5Z)-dodeca-3,5-dienoyl-[acp] + NADH + H+→a (5Z)-dodec-5-enoyl-[acp] + NAD+ (EC 1.3.1.9)
MPMX20_02485 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information