MetaCyc Pathway: methyl ketone biosynthesis (engineered) in Rhodopseudomonas palustris CGA009

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Reactions and Genes 4-Hydroxybenzoate (C)
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a fatty acid + ATP + coenzyme A→an acyl-CoA + AMP + diphosphate
No genes
Acyl-CoA oxidase:
a 2,3,4-saturated fatty acyl CoA + dioxygen→a (2E)-2-enoyl-CoA + hydrogen peroxide
(EC 1.3.3.6)
No genes
Enoyl-CoA hydratase (in reverse):
a (2E)-2-enoyl-CoA + H2O→a 2,3,4-saturated (3S)-3-hydroxyacyl-CoA
(EC 4.2.1.17)
TX73_001990 -0.1
TX73_002165 +0.0
TX73_002395 +0.0
TX73_002500 +0.1
TX73_002510 -0.0
TX73_003370 +0.4
TX73_003500 +0.0
TX73_004220 +0.2
TX73_004930 -0.2
TX73_006355 +0.1
TX73_007240 -0.0
TX73_008780 +0.1
TX73_008810 +0.0
TX73_009040 -0.1
TX73_009055 +0.0
TX73_009075 -0.1
TX73_009185 +0.1
TX73_009315 +1.5
TX73_011065 +0.4
TX73_011975 -0.2
TX73_014325 -0.0
TX73_017085 -0.1
TX73_017810 -0.1
TX73_017840 +2.3
TX73_017875 -0.0
TX73_019255 -0.1
TX73_019650 +0.0
TX73_022390 +0.0
TX73_022490 -0.3
3-hydroxyacyl-CoA dehydrogenase:
a 2,3,4-saturated (3S)-3-hydroxyacyl-CoA + NAD+→a 2,3,4-saturated 3-oxoacyl-CoA + NADH + H+
(EC 1.1.1.35)
TX73_004220 +0.2
TX73_008775 -0.0
TX73_011910 +0.2
TX73_018155 -0.1
TX73_019255 -0.1
a 2,3,4-saturated 3-oxoacyl-CoA + H2O→a 3-oxoacid + coenzyme A + H+
No genes
a 3-oxoacid + H+→a methyl ketone + CO2 (spontaneous)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information