MetaCyc Pathway: ammonia assimilation cycle III in Pseudomonas fluorescens FW300-N2C3

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Reactions and Genes L-Glutamine (C)
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Glutamate synthase (NADPH):
L-glutamine + H2O→ammonia + L-glutamate + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
AO356_01790 -0.0
AO356_03205 +0.2
AO356_03575 +0.0
AO356_04955 -0.3
AO356_04985 -0.3
AO356_05355 +0.2
AO356_05950 N.D.
AO356_06415 -0.8
AO356_06520 N.D.
AO356_07135 N.D.
AO356_07140 N.D.
AO356_07145 N.D.
AO356_07480 -2.9
AO356_07485 N.D.
AO356_09120 +0.1
AO356_09370 -0.0
AO356_09375 +0.1
AO356_14420 -5.0
AO356_14430 -5.3
AO356_14915 -0.1
AO356_17685 +0.1
AO356_18110 +0.0
AO356_18565 +0.0
AO356_18580 +0.0
AO356_19210 +0.2
AO356_20405 -0.8
AO356_20840 +0.1
AO356_21690 -3.9
AO356_23090 +0.1
AO356_25495 +0.0
AO356_26510 -0.3
AO356_28700 +0.0
AO356_30070 +0.0
AO356_30075 +0.0
Glutamate synthase (NADPH) (in reverse):
ammonia + 2-oxoglutarate + NADPH + 2 H+→L-glutamate + NADP+ + H2O (EC 1.4.1.13; 1.4.1.14)
AO356_01790 -0.0
AO356_03205 +0.2
AO356_04955 -0.3
AO356_04985 -0.3
AO356_05355 +0.2
AO356_06415 -0.8
AO356_09370 -0.0
AO356_09375 +0.1
AO356_14915 -0.1
AO356_19210 +0.2
AO356_23090 +0.1
AO356_25495 +0.0
AO356_28700 +0.0
AO356_30070 +0.0
AO356_30075 +0.0
Glutamate--ammonia ligase:
L-glutamate + ammonium + ATP→L-glutamine + ADP + H+ + phosphate (EC 6.3.1.2)
AO356_01510 +0.4
AO356_04435 +0.1
AO356_09705 N.D.
AO356_13135 +0.2
AO356_13145 +0.0
AO356_18875 -0.0
AO356_30085 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information