MetaCyc Pathway: stearate biosynthesis IV in Rhodanobacter denitrificans FW104-10B01

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Reactions and Genes R2A with nitrate 250 mM
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Beta-ketoacyl-acyl-carrier-protein synthase I:
a palmitoyl-[acp] + a malonyl-[acp] + H+→a 3-oxooctadecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2
(EC 2.3.1.41)
LRK54_RS09780 -1.2
LRK54_RS13915 -0.9
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxooctadecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxyoctadecanoyl-[acp] + NADP+
(EC 1.1.1.100)
LRK54_RS00850 -0.1
LRK54_RS01185 N.D.
LRK54_RS02570 -0.3
LRK54_RS08150 +0.0
LRK54_RS09760 -0.7
LRK54_RS14225 -0.1
LRK54_RS15975 +0.0
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyoctadecanoyl-[acp]→a (2E)-octadec-2-enoyl-[acp] + H2O
(EC 4.2.1.59)
LRK54_RS03305 N.D.
LRK54_RS13910 -1.2
a (2E)-octadec-2-enoyl-[acp] + NADPH + H+→a stearoyl-[acp] + NADP+ (EC 1.3.1.104)
No genes
Oleoyl-[acyl-carrier-protein] hydrolase:
a stearoyl-[acp] + H2O→stearate + a soluble [acyl-carrier protein] + H+
(EC 3.1.2.14)
No genes
Long-chain-fatty-acid--CoA ligase:
stearate + ATP + coenzyme A→stearoyl-CoA + AMP + diphosphate
(EC 6.2.1.3)
LRK54_RS00795 -0.7
LRK54_RS08400 +0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information