MetaCyc Pathway: L-valine degradation I in Pseudomonas fluorescens FW300-N2C3

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Reactions and Genes L-Lysine (C)
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Branched-chain-amino-acid transaminase:
L-valine + 2-oxoglutarate→3-methyl-2-oxobutanoate + L-glutamate (EC 2.6.1.42)
AO356_22970 -2.3
2-oxoisovalerate dehydrogenase (acylating):
3-methyl-2-oxobutanoate + coenzyme A + NAD+→isobutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
isobutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→methylacrylyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
AO356_26355 -0.1
methylacrylyl-CoA + H2O→(S)-3-hydroxy-isobutanoyl-CoA (EC 4.2.1.150)
AO356_02330 N.D.
AO356_04640 +0.0
AO356_10120 -0.0
AO356_12440 +0.0
AO356_17825 +0.2
AO356_25890 -0.4
AO356_26360 -0.8
AO356_26645 +0.1
AO356_30295 +0.2
AO356_30355 -0.3
AO356_30360 -0.2
3-hydroxyisobutyryl-CoA hydrolase:
(S)-3-hydroxy-isobutanoyl-CoA + H2O→(S)-3-hydroxy-isobutanoate + coenzyme A + H+ (EC 3.1.2.4)
AO356_26370 -0.1
3-hydroxyisobutyrate dehydrogenase:
(S)-3-hydroxy-isobutanoate + NAD+→(S)-methylmalonate-semialdehyde + NADH + H+ (EC 1.1.1.31)
AO356_02330 N.D.
AO356_07865 -0.9
AO356_10120 -0.0
AO356_20400 +0.0
AO356_30295 +0.2
(S)-3-amino-2-methylpropionate transaminase (in reverse):
(S)-methylmalonate-semialdehyde + L-glutamate→(S)-3-amino-2-methylpropanoate + 2-oxoglutarate (EC 2.6.1.22)
No genes
Methylmalonate-semialdehyde dehydrogenase (acylating):
(S)-methylmalonate-semialdehyde + coenzyme A + NAD+ + H2O→propanoyl-CoA + hydrogen carbonate + NADH + H+ (EC 1.2.1.27)
AO356_07870 -0.4
AO356_07950 -0.0
AO356_23175 +0.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information