MetaCyc Pathway: hexitol fermentation to lactate, formate, ethanol and acetate in Rahnella sp. WP5

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Reactions and Genes D-Mannitol (C)
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Mannitol-1-phosphate 5-dehydrogenase:
D-mannitol 1-phosphate + NAD+→β-D-fructofuranose 6-phosphate + NADH + H+ (EC 1.1.1.17)
EX31_RS05825 -3.9
Sorbitol-6-phosphate 2-dehydrogenase:
D-sorbitol 6-phosphate + NAD+→keto-D-fructose 6-phosphate + NADH + H+ (EC 1.1.1.140)
EX31_RS03345 -0.1
6-phosphofructokinase:
β-D-fructofuranose 6-phosphate + ATP→β-D-fructofuranose 1,6-bisphosphate + ADP + H+ (EC 2.7.1.11)
EX31_RS13510 +0.2
EX31_RS14310 -3.5
Fructose-bisphosphate aldolase:
β-D-fructofuranose 1,6-bisphosphate→glycerone phosphate + D-glyceraldehyde 3-phosphate (EC 4.1.2.13)
EX31_RS01425 +0.3
EX31_RS21415 N.D.
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
EX31_RS08670 -0.0
EX31_RS10785 +0.1
EX31_RS14275 +0.1
EX31_RS23635 -0.0
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
EX31_RS14300 N.D.
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
EX31_RS06280 N.D.
EX31_RS06915 +0.2
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
EX31_RS21410 N.D.
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
EX31_RS03970 -0.4
EX31_RS14360 +0.1
EX31_RS23555 N.D.
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.11)
EX31_RS23555 N.D.
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
EX31_RS07940 N.D.
Pyruvate, water dikinase:
pyruvate + ATP + H2O→phosphoenolpyruvate + AMP + 2 H+ + phosphate (EC 2.7.9.2)
EX31_RS13935 -2.7
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
EX31_RS10640 +0.2
EX31_RS13870 -0.9
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
EX31_RS09825 -0.2
EX31_RS09830 -0.2
EX31_RS14675 -0.1
EX31_RS14680 +0.1
EX31_RS18270 -0.2
EX31_RS18470 -0.6
EX31_RS21780 +0.1
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
EX31_RS10075 -0.1
L-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(S)-lactate + NAD+ (EC 1.1.1.27)
EX31_RS22840 -0.2
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
EX31_RS19485 -0.5
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
EX31_RS19480 -0.3
EX31_RS24735 -0.6
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
EX31_RS00895 +0.2
EX31_RS08040 +0.1
EX31_RS10075 -0.1
EX31_RS10950 -0.1
EX31_RS15570 -0.1
EX31_RS16670 +0.2
EX31_RS18715 +0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information