MetaCyc Pathway: superpathway of fatty acid biosynthesis I (E. coli) in Burkholderia phytofirmans PsJN

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Reactions and Genes inner cut, LB soft agar motility assay
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[Acyl-carrier-protein] S-acetyltransferase:
acetyl-CoA + a soluble [acyl-carrier protein]→an acetyl-[acp] + coenzyme A
(EC 2.3.1.38)
No genes
acetyl-CoA + a [carboxyl-carrier protein dimer]-N6-carboxybiotinyl-L-lysine→malonyl-CoA + a [biotin carboxyl-carrier-protein dimer]-N6-biotinyl-L-lysine (EC 2.1.3.15)
BPHYT_RS12420 N.D.
BPHYT_RS33860 N.D.
[Acyl-carrier-protein] S-malonyltransferase:
malonyl-CoA + a soluble [acyl-carrier protein]→a malonyl-[acp] + coenzyme A
(EC 2.3.1.39)
BPHYT_RS14430 N.D.
BPHYT_RS23905 -0.4
Beta-ketoacyl-acyl-carrier-protein synthase III:
acetyl-CoA + a malonyl-[acp] + H+→an acetoacetyl-[acp] + CO2 + coenzyme A
(EC 2.3.1.180)
BPHYT_RS00625 -0.1
BPHYT_RS00655 +0.0
BPHYT_RS14435 N.D.
BPHYT_RS19110 -1.3
BPHYT_RS19120 -2.6
BPHYT_RS30955 +0.2
Beta-ketoacyl-acyl-carrier-protein synthase II:
an acetyl-[acp] + a malonyl-[acp] + H+→an acetoacetyl-[acp] + a soluble [acyl-carrier protein] + CO2
(EC 2.3.1.179; 2.3.1.41)
BPHYT_RS14415 N.D.
BPHYT_RS15125 -0.2
BPHYT_RS15130 -0.1
BPHYT_RS15145 -0.3
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+→a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
(EC 1.1.1.100)
BPHYT_RS01050 +0.2
BPHYT_RS04770 -0.2
BPHYT_RS05365 +0.1
BPHYT_RS05385 -0.3
BPHYT_RS06420 N.D.
BPHYT_RS07065 -0.2
BPHYT_RS08285 N.D.
BPHYT_RS08365 -0.3
BPHYT_RS08890 +0.2
BPHYT_RS10170 +0.2
BPHYT_RS10200 N.D.
BPHYT_RS10595 -0.5
BPHYT_RS10630 -0.1
BPHYT_RS10735 N.D.
BPHYT_RS11845 N.D.
BPHYT_RS13055 -0.4
BPHYT_RS14425 N.D.
BPHYT_RS15135 +0.5
BPHYT_RS15890 +0.0
BPHYT_RS16040 N.D.
BPHYT_RS16940 +0.3
BPHYT_RS20510 N.D.
BPHYT_RS20625 +0.6
BPHYT_RS20775 +0.2
BPHYT_RS21600 -0.0
BPHYT_RS22135 -0.4
BPHYT_RS25030 +0.3
BPHYT_RS25415 N.D.
BPHYT_RS25655 +0.1
BPHYT_RS26470 -0.8
BPHYT_RS28250 N.D.
BPHYT_RS30440 +0.4
BPHYT_RS30465 +0.6
BPHYT_RS30795 +2.7
BPHYT_RS31385 -0.1
BPHYT_RS31405 N.D.
BPHYT_RS31610 -0.1
BPHYT_RS31900 -0.2
BPHYT_RS32940 -0.1
BPHYT_RS33140 N.D.
BPHYT_RS33705 +0.5
BPHYT_RS34945 N.D.
BPHYT_RS34965 N.D.
BPHYT_RS35505 +0.1
BPHYT_RS35675 -1.0
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyacyl-[acyl-carrier protein]→a trans-2-enoyl-[acyl-carrier protein] + H2O
(EC 4.2.1.59)
BPHYT_RS12085 N.D.
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxydecanoyl-[acp]→a (2E)-dec-2-enoyl-[acp] + H2O
(EC 4.2.1.59)
BPHYT_RS12085 N.D.
Trans-2-decenoyl-[acyl-carrier-protein] isomerase:
a (2E)-dec-2-enoyl-[acp]→a (3Z)-dec-3-enoyl-[acp]
(EC 5.3.3.14)
No genes
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a trans-2-enoyl-[acyl-carrier protein] + NADH + H+→a 2,3,4-saturated fatty acyl-[acp] + NAD+
(EC 1.3.1.9)
BPHYT_RS01880 +0.1
BPHYT_RS12390 +0.0
BPHYT_RS20615 +0.2
BPHYT_RS30015 N.D.
BPHYT_RS33380 +0.0
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a trans-2-enoyl-[acyl-carrier protein] + NADPH + H+→a 2,3,4-saturated fatty acyl-[acp] + NADP+
(EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
BPHYT_RS12390 +0.0
BPHYT_RS32935 -0.2
Beta-ketoacyl-acyl-carrier-protein synthase II:
a 2,3,4-saturated fatty acyl-[acp] + a malonyl-[acp] + H+→a 3-oxoacyl-[acyl-carrier protein] + a soluble [acyl-carrier protein] + CO2
(EC 2.3.1.179; 2.3.1.41)
BPHYT_RS14415 N.D.
BPHYT_RS15125 -0.2
BPHYT_RS15130 -0.1
BPHYT_RS15145 -0.3
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (3Z)-dec-3-enoyl-[acp] + a malonyl-[acp] + H+→a (5Z)-3-oxododec-5-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2
(EC 2.3.1.179; 2.3.1.41)
BPHYT_RS14415 N.D.
BPHYT_RS15125 -0.2
BPHYT_RS15130 -0.1
BPHYT_RS15145 -0.3
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (5Z)-3-oxododec-5-enoyl-[acp] + NADPH + H+→a (3R,5Z)-3-hydroxydodec-5-enoyl-[acp] + NADP+
(EC 1.1.1.100)
BPHYT_RS01050 +0.2
BPHYT_RS04770 -0.2
BPHYT_RS05365 +0.1
BPHYT_RS05385 -0.3
BPHYT_RS06420 N.D.
BPHYT_RS07065 -0.2
BPHYT_RS08285 N.D.
BPHYT_RS08365 -0.3
BPHYT_RS08890 +0.2
BPHYT_RS10170 +0.2
BPHYT_RS10200 N.D.
BPHYT_RS10595 -0.5
BPHYT_RS10630 -0.1
BPHYT_RS10735 N.D.
BPHYT_RS11845 N.D.
BPHYT_RS13055 -0.4
BPHYT_RS14425 N.D.
BPHYT_RS15135 +0.5
BPHYT_RS15890 +0.0
BPHYT_RS16040 N.D.
BPHYT_RS16940 +0.3
BPHYT_RS20510 N.D.
BPHYT_RS20625 +0.6
BPHYT_RS20775 +0.2
BPHYT_RS21600 -0.0
BPHYT_RS22135 -0.4
BPHYT_RS25030 +0.3
BPHYT_RS25415 N.D.
BPHYT_RS25655 +0.1
BPHYT_RS26470 -0.8
BPHYT_RS28250 N.D.
BPHYT_RS30440 +0.4
BPHYT_RS30465 +0.6
BPHYT_RS30795 +2.7
BPHYT_RS31385 -0.1
BPHYT_RS31405 N.D.
BPHYT_RS31610 -0.1
BPHYT_RS31900 -0.2
BPHYT_RS33140 N.D.
BPHYT_RS33705 +0.5
BPHYT_RS34945 N.D.
BPHYT_RS34965 N.D.
BPHYT_RS35505 +0.1
BPHYT_RS35675 -1.0
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,5Z)-3-hydroxydodec-5-enoyl-[acp]→a (2E,5Z)-dodeca-3,5-dienoyl-[acp] + H2O
(EC 4.2.1.59)
BPHYT_RS12085 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,5Z)-dodeca-3,5-dienoyl-[acp] + NADH + H+→a (5Z)-dodec-5-enoyl-[acp] + NAD+
(EC 1.3.1.9)
BPHYT_RS01880 +0.1
BPHYT_RS12390 +0.0
BPHYT_RS20615 +0.2
BPHYT_RS30015 N.D.
BPHYT_RS33380 +0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information