MetaCyc Pathway: trans-4-hydroxy-L-proline degradation II in Pseudomonas fluorescens FW300-N2C3

Reactions and Genes	D-Trehalose (C)
4-hydroxyproline epimerase: trans-4-hydroxy-L-proline→(4R)-4-hydroxy-D-proline (EC 5.1.1.8)
AO356_09240	-0.0
AO356_24960	+0.1
(4R)-4-hydroxy-D-proline + a reduced two electron carrier + dioxygen→1-pyrroline-4-hydroxy-2-carboxylate + an oxidized electron carrier + H+ + 2 H2O
AO356_24945	+0.0
AO356_24955	+0.0
1-pyrroline-4-hydroxy-2-carboxylate deaminase: 1-pyrroline-4-hydroxy-2-carboxylate + H+ + H2O→2,5-dioxopentanoate + ammonium (EC 3.5.4.22)
AO356_24905	-1.6
2,5-dioxovalerate dehydrogenase: 2,5-dioxopentanoate + NADP+ + H2O→2-oxoglutarate + NADPH + 2 H+ (EC 1.2.1.26)
AO356_07180	+0.1
AO356_24600	-0.1
AO356_27465	+0.0

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information