MetaCyc Pathway: heterolactic fermentation in Pseudomonas fluorescens FW300-N2C3

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Reactions and Genes D-Sorbitol (C)
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Fructokinase:
β-D-fructofuranose + ATP→β-D-fructofuranose 6-phosphate + ADP + H+ (EC 2.7.1.4)
AO356_00035 -5.0
AO356_27700 -0.2
Hexokinase:
D-glucopyranose + ATP→D-glucopyranose 6-phosphate + ADP + H+ (EC 2.7.1.1; 2.7.1.2)
AO356_05215 -0.3
Glucose-6-phosphate isomerase (in reverse):
β-D-fructofuranose 6-phosphate→α-D-glucose 6-phosphate (EC 5.3.1.9)
AO356_16050 -3.5
AO356_23525 +0.1
Glucose-6-phosphate dehydrogenase:
D-glucopyranose 6-phosphate + NADP+→6-phospho D-glucono-1,5-lactone + NADPH + H+ (EC 1.1.1.49)
AO356_05160 -4.1
AO356_27850 +0.2
6-phosphogluconolactonase:
6-phospho D-glucono-1,5-lactone + H2O→D-gluconate 6-phosphate + H+ (EC 3.1.1.31)
AO356_05155 N.D.
AO356_30310 -0.2
D-gluconate 6-phosphate + NAD(P)+→CO2 + D-ribulose 5-phosphate + NAD(P)H (EC 1.1.1.351)
No genes
Ribulose-phosphate 3-epimerase:
D-ribulose 5-phosphate→D-xylulose 5-phosphate (EC 5.1.3.1)
AO356_14410 N.D.
Phosphoketolase:
D-xylulose 5-phosphate + phosphate→acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O (EC 4.1.2.9)
AO356_23915 -0.1
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
AO356_02265 -0.6
AO356_05225 -3.2
Phosphate acetyltransferase (in reverse):
acetyl phosphate + coenzyme A→acetyl-CoA + phosphate (EC 2.3.1.8)
AO356_16680 +0.0
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
AO356_28020 -0.0
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
AO356_13690 N.D.
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
AO356_00820 +0.1
AO356_04765 +0.0
AO356_05920 +0.1
AO356_14405 -0.1
AO356_20305 -0.0
AO356_28020 -0.0
AO356_29985 -0.3
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.11)
No genes
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
AO356_05940 N.D.
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
AO356_17830 -2.1
AO356_19600 +0.2
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
AO356_19040 +0.0
L-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(S)-lactate + NAD+ (EC 1.1.1.27)
AO356_07560 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information