MetaCyc Pathway: L-isoleucine biosynthesis III in Pseudomonas fluorescens FW300-N2C3

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Reactions and Genes L-Lysine (C)
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Methylaspartate mutase (in reverse):
L-glutamate→(2S, 3S)-3-methylaspartate (EC 5.4.99.1)
AO356_18625 -0.2
(2S, 3S)-3-methylaspartate + 2-oxoglutarate→L-glutamate + 2-methyl-3-oxosuccinate
No genes
2-methyl-3-oxosuccinate + H+→2-oxobutanoate + CO2
No genes
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
AO356_16170 -3.7
AO356_16175 -3.6
AO356_17755 -0.3
AO356_28690 -0.1
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
AO356_16180 -3.7
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
AO356_13250 -4.1
AO356_24585 +0.0
AO356_28760 +0.2
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
AO356_22970 -2.5

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information