MetaCyc Pathway: mixed acid fermentation in Pseudomonas fluorescens FW300-N2C3

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Reactions and Genes L-Lysine (C)
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Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate (EC 4.1.1.31)
AO356_06185 -0.2
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
AO356_17830 -0.9
AO356_19600 +0.4
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
AO356_19040 -0.0
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
No genes
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
No genes
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
AO356_28020 +0.2
Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
AO356_19670 N.D.
formate + H+→CO2 + H2
No genes
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
AO356_03545 N.D.
AO356_07075 -0.3
AO356_17810 N.D.
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
AO356_16680 -1.3
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
AO356_17605 +0.1
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
AO356_02385 +0.0
AO356_20875 -1.5
AO356_24390 N.D.
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
AO356_00820 -0.1
AO356_04765 -0.0
AO356_05920 -0.3
AO356_14405 -0.2
AO356_20305 -0.1
AO356_28020 +0.2
AO356_29985 -0.5
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
No genes
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
AO356_02385 +0.0
AO356_20875 -1.5
AO356_24390 N.D.
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→CO2 + 2-oxoglutarate + NADPH (EC 1.1.1.42)
AO356_22125 -1.3
AO356_22135 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information