MetaCyc Pathway: dTDP-α-D-olivose, dTDP-α-D-oliose and dTDP-α-D-mycarose biosynthesis in Sinorhizobium meliloti 1021

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Reactions and Genes Fusidic 0.0001 mg/ml
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Glucose-1-phosphate thymidylyltransferase:
α-D-glucopyranose 1-phosphate + H+ + dTTP→dTDP-α-D-glucose + diphosphate
(EC 2.7.7.24)
SM_b21324 +0.0
dTDP-glucose 4,6-dehydratase:
dTDP-α-D-glucose→dTDP-4-dehydro-6-deoxy-α-D-glucopyranose + H2O
(EC 4.2.1.46)
SM_b20239 -0.1
SM_b20458 -0.0
SM_b21052 -0.0
SM_b21326 -0.1
SM_b21426 -1.1
dTDP-4-dehydro-6-deoxy-α-D-glucopyranose→dTDP-2,6-dideoxy-D-glycero-hex-2-enos-4-ulose + H+ + H2O (EC 4.2.1.159)
No genes
dTDP-2,6-dideoxy-D-glycero-hex-2-enos-4-ulose + H+→dTDP-3,4-didehydro-2,6-dideoxy-α-D-glucose (spontaneous) (EC 4.2.1.159)
No genes
dTDP-3,4-didehydro-2,6-dideoxy-α-D-glucose + NADPH + H+→dTDP-4-dehydro-2,6-dideoxy-α-D-threo-hexopyranose + NADP+ (EC 1.1.1.384)
No genes
dTDP-4-dehydro-2,6-dideoxy-α-D-threo-hexopyranose + NADPH + H+→dTDP-α-D-olivose + NADP+
SM_b21418 -1.3
dTDP-4-dehydro-2,6-dideoxy-α-D-threo-hexopyranose + NADPH + H+→dTDP-α-D-oliose + NADP+
No genes
dTDP-4-dehydro-2,6-dideoxy-α-D-threo-hexopyranose + S-adenosyl-L-methionine→dTDP-4-dehydro-3-C-methyl-2,6-dideoxy-α-D-glucose + S-adenosyl-L-homocysteine + H+
SM_b21418 -1.3
dTDP-4-dehydro-3-C-methyl-2,6-dideoxy-α-D-glucose + NADPH + H+→dTDP-α-D-mycarose + NADP+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information