MetaCyc Pathway: superpathway of L-methionine biosynthesis (by sulfhydrylation) in Pseudomonas fluorescens FW300-N2C3

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Reactions and Genes Thallium(I) acetate 0.008 mg/ml
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
AO356_01275 -0.0
AO356_03090 -0.2
AO356_03985 -0.1
AO356_06565 -0.1
AO356_12070 -0.1
AO356_12595 +0.1
AO356_16875 -0.3
AO356_19495 -0.1
AO356_19865 +0.1
AO356_19905 -0.1
AO356_20345 -0.4
AO356_22880 +0.1
AO356_24005 +0.9
AO356_24115 -0.1
AO356_24720 -0.2
AO356_24975 +0.0
AO356_25355 -0.1
Sulfate adenylyltransferase:
sulfate + ATP + H+→adenosine 5'-phosphosulfate + diphosphate (EC 2.7.7.4)
AO356_16665 +0.1
AO356_16670 -0.1
Adenylyl-sulfate kinase:
adenosine 5'-phosphosulfate + ATP→3'-phosphoadenylyl-sulfate + ADP + H+ (EC 2.7.1.25)
AO356_08855 -0.2
AO356_16670 -0.1
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
AO356_18775 -0.4
Phosphoadenylyl-sulfate reductase (thioredoxin) (in reverse):
3'-phosphoadenylyl-sulfate + a reduced thioredoxin→sulfite + adenosine 3',5'-bisphosphate + an oxidized thioredoxin + 2 H+ (EC 1.8.4.8)
AO356_20830 +1.3
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
AO356_04970 -0.6
AO356_21420 N.D.
AO356_21425 +0.1
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
AO356_06375 N.D.
AO356_15140 -0.8
AO356_27140 +1.5
Sulfite reductase (NADPH) (in reverse):
sulfite + 3 NADPH + 5 H+→hydrogen sulfide + 3 NADP+ + 3 H2O (EC 1.8.1.2)
AO356_00555 -0.4
AO356_07315 -0.0
Homoserine O-acetyltransferase:
L-homoserine + acetyl-CoA→O-acetyl-L-homoserine + coenzyme A (EC 2.3.1.31)
AO356_13405 -0.0
AO356_13410 -0.1
O-acetylhomoserine aminocarboxypropyltransferase:
O-acetyl-L-homoserine + hydrogen sulfide→L-homocysteine + acetate + H+ (EC 2.5.1.49)
AO356_21470 +0.2
AO356_29805 +0.2
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase:
L-homocysteine + 5-methyltetrahydropteroyl tri-L-glutamate→L-methionine + tetrahydropteroyl tri-L-glutamate (EC 2.1.1.14)
AO356_17740 -0.0
Methionine synthase:
L-homocysteine + a 5-methyltetrahydrofolate→L-methionine + a tetrahydrofolate (EC 2.1.1.13)
AO356_00545 +0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information