MetaCyc Pathway: L-isoleucine biosynthesis I (from threonine) in Paraburkholderia graminis OAS925

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Reactions and Genes D-Serine (C)
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Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
ABIE53_000761 -0.0
ABIE53_002093 -0.1
ABIE53_002293 -1.4
ABIE53_003253 -0.1
ABIE53_004296 -0.9
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
ABIE53_000761 -0.0
ABIE53_002093 -0.1
ABIE53_002293 -1.4
ABIE53_003253 -0.1
ABIE53_004296 -0.9
ABIE53_005908 +0.2
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
ABIE53_000761 -0.0
ABIE53_002093 -0.1
ABIE53_002293 -1.4
ABIE53_003253 -0.1
ABIE53_004296 -0.9
ABIE53_005908 +0.2
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
ABIE53_000241 +0.3
ABIE53_001436 +0.0
ABIE53_001574 N.D.
ABIE53_001575 -1.1
ABIE53_002578 +0.0
ABIE53_002579 +0.7
ABIE53_005071 +0.1
ABIE53_005846 -0.0
ABIE53_006320 -0.2
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
ABIE53_001576 N.D.
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
ABIE53_000325 -0.3
ABIE53_001239 -0.0
ABIE53_005331 -0.1
ABIE53_006253 -0.1
ABIE53_006513 -0.5
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
ABIE53_003432 +0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information