MetaCyc Pathway: L-isoleucine biosynthesis V in Rhodospirillum rubrum S1H

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Reactions and Genes carbon source anaerobic Succinic Acid 31.25 mM
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Acetate--CoA ligase:
2-methylbutanoate + ATP + coenzyme A→(2S)-2-methylbutanoyl-CoA + AMP + diphosphate (EC 6.2.1.1)
Rru_A1046 +0.3
Rru_A3109 -0.2
Rru_A3575 -1.9
3-methyl-2-oxobutanoate dehydrogenase (ferredoxin) (in reverse):
(2S)-2-methylbutanoyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→(3S)-3-methyl-2-oxopentanoate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.7)
No genes
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
Rru_A0300 +0.5
Rru_A0508 +0.4
Rru_A1131 -0.3
Rru_A2223 -0.5

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information