MetaCyc Pathway: 2-methyl-branched fatty acid β-oxidation in Pseudomonas fluorescens FW300-N2E3

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Reactions and Genes Tween 20 (C)
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Butyrate--CoA ligase:
a short-chain 2-methyl-branched fatty acid + ATP + coenzyme A→a short-chain 2-methyl fatty acyl-CoA + AMP + diphosphate (EC 6.2.1.2)
No genes
a long-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a long-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)
AO353_15225 -0.1
Butyrate--CoA ligase:
a medium-chain 2-methyl-branched fatty acid + ATP + coenzyme A→a medium-chain 2-methyl fatty acyl-CoA + AMP + diphosphate (EC 6.2.1.2)
No genes
a short-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a short-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
AO353_25670 +0.0
AO353_25680 -0.2
a medium-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a medium-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7)
AO353_06340 +0.0
AO353_07485 -1.8
AO353_07495 -3.6
AO353_12805 +0.1
AO353_15225 -0.1
AO353_21065 -0.1
Enoyl-CoA hydratase (in reverse):
a long-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a long-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
AO353_00510 -3.4
AO353_01135 -0.4
AO353_01155 +0.0
AO353_03780 +0.0
AO353_17555 -0.0
AO353_19125 N.D.
AO353_23055 -0.2
AO353_24830 -0.1
AO353_25665 -0.3
AO353_25675 N.D.
AO353_26540 -0.2
Enoyl-CoA hydratase (in reverse):
a short-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a short-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
AO353_00510 -3.4
AO353_01135 -0.4
AO353_01155 +0.0
AO353_03780 +0.0
AO353_17555 -0.0
AO353_19125 N.D.
AO353_23055 -0.2
AO353_24830 -0.1
AO353_25665 -0.3
AO353_25675 N.D.
AO353_26540 -0.2
Enoyl-CoA hydratase (in reverse):
a medium-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a medium-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
AO353_00510 -3.4
AO353_01135 -0.4
AO353_01155 +0.0
AO353_03780 +0.0
AO353_17555 -0.0
AO353_19125 N.D.
AO353_23055 -0.2
AO353_24830 -0.1
AO353_25665 -0.3
AO353_25675 N.D.
AO353_26540 -0.2
3-hydroxyacyl-CoA dehydrogenase:
a long-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a long-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.35)
AO353_00510 -3.4
AO353_01155 +0.0
3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
a short-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a short-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.178)
No genes
3-hydroxyacyl-CoA dehydrogenase:
a medium-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a medium-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.35)
AO353_00510 -3.4
AO353_01155 +0.0
Acetyl-CoA C-acyltransferase (in reverse):
a long-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a long-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
AO353_00505 -3.6
AO353_01065 +0.3
AO353_01160 -0.4
AO353_05785 +0.2
AO353_13880 -0.0
AO353_17205 -0.8
AO353_25685 -0.0
AO353_27530 -0.2
AO353_27945 -0.1
Acetyl-CoA C-acyltransferase (in reverse):
a short-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a short-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
AO353_00505 -3.6
AO353_01065 +0.3
AO353_01160 -0.4
AO353_05785 +0.2
AO353_13880 -0.0
AO353_17205 -0.8
AO353_25685 -0.0
AO353_27530 -0.2
AO353_27945 -0.1
Acetyl-CoA C-acyltransferase (in reverse):
a medium-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a medium-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
AO353_00505 -3.6
AO353_01065 +0.3
AO353_01160 -0.4
AO353_05785 +0.2
AO353_13880 -0.0
AO353_17205 -0.8
AO353_25685 -0.0
AO353_27530 -0.2
AO353_27945 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information