MetaCyc Pathway: nitrogen remobilization from senescing leaves in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes octanoate (C)
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Pyruvate, phosphate dikinase:
pyruvate + ATP + phosphate→phosphoenolpyruvate + AMP + diphosphate + H+
(EC 2.7.9.1)
No genes
Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate
(EC 4.1.1.31)
Pf6N2E2_3103 -1.3
Citrate (Si)-synthase:
oxaloacetate + acetyl-CoA + H2O→citrate + coenzyme A + H+
(EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
Pf6N2E2_5850 N.D.
Aconitate hydratase:
citrate→cis-aconitate + H2O
(EC 4.2.1.3)
Pf6N2E2_2301 -0.1
Pf6N2E2_564 N.D.
Pf6N2E2_6063 -0.9
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate
(EC 4.2.1.3)
Pf6N2E2_2301 -0.1
Pf6N2E2_564 N.D.
Pf6N2E2_6063 -0.9
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→2-oxoglutarate + CO2 + NADPH
(EC 1.1.1.42)
Pf6N2E2_288 -4.2
Pf6N2E2_289 -0.8
Isocitrate--homoisocitrate dehydrogenase:
D-threo-isocitrate + NAD+→2-oxoglutarate + CO2 + NADH
(EC 1.1.1.286; 1.1.1.41)
No genes
Glutamate synthase (ferredoxin) (in reverse):
2-oxoglutarate + L-glutamine + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→2 L-glutamate + 2 an oxidized ferredoxin [iron-sulfur] cluster
(EC 1.4.7.1)
Pf6N2E2_5461 -0.7

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information