MetaCyc Pathway: mixed acid fermentation in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes octanoate (C)
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Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate (EC 4.1.1.31)
Pf6N2E2_3103 -1.3
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
Pf6N2E2_5494 -2.5
Pf6N2E2_5835 -0.5
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
Pf6N2E2_5719 -0.6
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
No genes
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
No genes
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
Pf6N2E2_1380 N.D.
Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
Pf6N2E2_5850 N.D.
formate + H+→CO2 + H2
No genes
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
Pf6N2E2_2536 -1.1
Pf6N2E2_3276 -0.1
Pf6N2E2_5489 N.D.
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
Pf6N2E2_5260 +0.3
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
Pf6N2E2_5444 +0.5
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
Pf6N2E2_2301 -0.1
Pf6N2E2_564 N.D.
Pf6N2E2_6063 -0.9
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
Pf6N2E2_1380 N.D.
Pf6N2E2_1522 +0.1
Pf6N2E2_194 +0.3
Pf6N2E2_2802 -0.0
Pf6N2E2_3044 -0.1
Pf6N2E2_4819 +0.0
Pf6N2E2_5980 -0.0
Pf6N2E2_840 +0.1
Pf6N2E2_999 -0.4
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
No genes
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
Pf6N2E2_2301 -0.1
Pf6N2E2_564 N.D.
Pf6N2E2_6063 -0.9
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→CO2 + 2-oxoglutarate + NADPH (EC 1.1.1.42)
Pf6N2E2_288 -4.2
Pf6N2E2_289 -0.8

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information