MetaCyc Pathway: butanol and isobutanol biosynthesis (engineered) in Azospirillum brasilense Sp245

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Reactions and Genes nitrate (N)
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Glycine oxidase:
glycine + dioxygen + H2O→glyoxylate + ammonium + hydrogen peroxide (EC 1.4.3.19; 1.4.3.3)
AZOBR_RS30970 -1.4
3-ethylmalate synthase:
glyoxylate + butanoyl-CoA + H2O→3-ethylmalate + coenzyme A + H+ (EC 2.3.3.7)
No genes
3-isopropylmalate dehydrogenase:
3-ethylmalate + NAD+→2-oxovalerate + CO2 + NADH (EC 1.1.1.85)
AZOBR_RS14130 -3.0
2-oxovalerate→3-methyl-2-oxobutanoate
No genes
Pyruvate decarboxylase:
2-oxovalerate + H+→1-butanal + CO2 (EC 4.1.1.1)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
AZOBR_RS06065 -0.3
AZOBR_RS10415 -0.6
AZOBR_RS13230 -0.2
AZOBR_RS16515 +0.0
AZOBR_RS20975 +0.0
AZOBR_RS26265 -0.2
AZOBR_RS26520 +0.8
AZOBR_RS27605 -0.2
AZOBR_RS28580 -0.0
AZOBR_RS32240 +0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information