MetaCyc Pathway: glutaminyl-tRNAgln biosynthesis via transamidation in Shewanella sp. ANA-3

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Reactions and Genes L-Glutamine (N)
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Glutamate synthase (NADPH):
L-glutamine + H2O→ammonia + L-glutamate + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
Shewana3_0265 -0.3
Shewana3_0296 -0.1
Shewana3_0606 +0.3
Shewana3_0753 -0.2
Shewana3_0890 +1.2
Shewana3_0967 -6.6
Shewana3_0968 (carB) -6.6
Shewana3_0974 +0.3
Shewana3_1115 (pyrG) N.D.
Shewana3_1184 -4.6
Shewana3_1209 +0.3
Shewana3_1236 (guaA) -4.9
Shewana3_1311 +0.1
Shewana3_1519 -6.8
Shewana3_1520 -7.6
Shewana3_1696 +0.6
Shewana3_2303 -0.3
Shewana3_2549 (asnB) -8.9
Shewana3_2598 N.D.
Shewana3_2851 +0.1
Shewana3_3050 (gltB) -3.5
Shewana3_3051 (gltD) -3.6
Shewana3_3523 -0.1
Shewana3_3782 +0.2
Shewana3_3808 +0.1
Shewana3_3865 +0.4
Shewana3_3866 -0.1
Shewana3_3952 +1.7
Glutamate--tRNA(Gln) ligase:
L-glutamate + ATP + a tRNAGln→an L-glutamyl-[tRNAGln] + AMP + diphosphate (EC 6.1.1.24)
No genes
Glutaminyl-tRNA synthase (glutamine-hydrolyzing):
an L-glutamyl-[tRNAGln] + ATP→a 5-phosphooxy-L-glutamyl-[tRNAGln] + ADP (EC 6.3.5.7)
No genes
Glutaminyl-tRNA synthase (glutamine-hydrolyzing):
a 5-phosphooxy-L-glutamyl-[tRNAGln] + ammonia→an L-glutaminyl-[tRNAGln] + phosphate (EC 6.3.5.7)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information