MetaCyc Pathway: L-isoleucine degradation I in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes L-Isoleucine (C)
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Branched-chain-amino-acid transaminase:
L-isoleucine + 2-oxoglutarate→(3S)-3-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42)
Pf6N2E2_477 -5.5
2-oxoisovalerate dehydrogenase (acylating):
(3S)-3-methyl-2-oxopentanoate + coenzyme A + NAD+→(2S)-2-methylbutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
(2S)-2-methylbutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(E)-2-methylcrotonoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
Pf6N2E2_1146 -2.1
(E)-2-methylcrotonoyl-CoA + H2O→(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA (EC 4.2.1.150)
Pf6N2E2_1013 -0.2
Pf6N2E2_1046 -0.1
Pf6N2E2_1834 +0.5
Pf6N2E2_1835 -0.3
Pf6N2E2_1840 +0.4
Pf6N2E2_1850 +0.1
Pf6N2E2_1922 -0.0
Pf6N2E2_1934 -0.4
Pf6N2E2_1935 -0.2
Pf6N2E2_2290 N.D.
Pf6N2E2_2773 +0.2
Pf6N2E2_3894 -0.1
Pf6N2E2_5493 -0.1
Pf6N2E2_6000 +0.1
3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+→2-methylacetoacetyl-CoA + NADH + H+ (EC 1.1.1.178)
Pf6N2E2_1144 N.D.
Pf6N2E2_1833 +0.3
Pf6N2E2_1850 +0.1
Pf6N2E2_1922 -0.0
Pf6N2E2_2290 N.D.
Pf6N2E2_3894 -0.1
Pf6N2E2_6000 +0.1
Acetyl-CoA C-acyltransferase (in reverse):
2-methylacetoacetyl-CoA + coenzyme A→acetyl-CoA + propanoyl-CoA (EC 2.3.1.16)
Pf6N2E2_1145 N.D.
Pf6N2E2_1675 -0.2
Pf6N2E2_1836 +0.1
Pf6N2E2_1851 +0.0
Pf6N2E2_2113 -0.2
Pf6N2E2_2289 N.D.
Pf6N2E2_2539 -0.0
Pf6N2E2_2835 -0.3
Pf6N2E2_3492 -0.2
Pf6N2E2_5999 -0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information