MetaCyc Pathway: L-isoleucine biosynthesis II in Azospirillum brasilense Sp245

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Reactions and Genes L-Leucine (N)
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pyruvate + acetyl-CoA + H2O→(R)-citramalate + coenzyme A + H+ (EC 2.3.3.21)
No genes
(R)-2-methylmalate dehydratase:
(R)-citramalate→citraconate + H2O (EC 4.2.1.35)
AZOBR_RS03545 -0.2
AZOBR_RS14135 +0.1
AZOBR_RS15560 N.D.
AZOBR_RS15565 -0.1
AZOBR_RS27810 N.D.
AZOBR_RS27815 +0.2
citraconate + H2O→(2R,3S)-3-methylmalate
AZOBR_RS14135 +0.1
AZOBR_RS15560 N.D.
AZOBR_RS15565 -0.1
AZOBR_RS27815 +0.2
(2R,3S)-3-methylmalate + NAD+→2-oxobutanoate + CO2 + NADH
AZOBR_RS14130 -0.8
Acetolactate synthase:
2-oxobutanoate + pyruvate + H+→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
AZOBR_RS06560 -0.6
AZOBR_RS06565 -0.4
AZOBR_RS23050 -0.2
(S)-2-aceto-2-hydroxybutanoate + NAD(P)H + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NAD(P)+ (EC 1.1.1.383)
No genes
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
AZOBR_RS15015 +0.5
AZOBR_RS31260 -0.1
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
AZOBR_RS06555 -2.3
AZOBR_RS16425 -0.1
AZOBR_RS23800 -0.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information