MetaCyc Pathway: L-isoleucine degradation I in Azospirillum brasilense Sp245

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Reactions and Genes L-Leucine (N)
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Branched-chain-amino-acid transaminase:
L-isoleucine + 2-oxoglutarate→(3S)-3-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42)
AZOBR_RS06555 -2.3
AZOBR_RS16425 -0.1
AZOBR_RS23800 -0.4
2-oxoisovalerate dehydrogenase (acylating):
(3S)-3-methyl-2-oxopentanoate + coenzyme A + NAD+→(2S)-2-methylbutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
AZOBR_RS14390 N.D.
(2S)-2-methylbutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(E)-2-methylcrotonoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
No genes
(E)-2-methylcrotonoyl-CoA + H2O→(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA (EC 4.2.1.150)
AZOBR_RS01260 -0.1
AZOBR_RS03680 +0.0
AZOBR_RS07775 -0.1
AZOBR_RS09775 N.D.
AZOBR_RS13325 +0.2
AZOBR_RS15730 -0.6
AZOBR_RS18155 +0.3
AZOBR_RS20225 -0.1
AZOBR_RS24105 +0.3
AZOBR_RS24715 -0.6
AZOBR_RS26270 +0.2
AZOBR_RS26485 -0.2
AZOBR_RS26830 +0.1
AZOBR_RS27635 -0.6
AZOBR_RS29210 -0.1
3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+→2-methylacetoacetyl-CoA + NADH + H+ (EC 1.1.1.178)
AZOBR_RS09555 -0.1
AZOBR_RS20225 -0.1
AZOBR_RS26110 N.D.
Acetyl-CoA C-acyltransferase (in reverse):
2-methylacetoacetyl-CoA + coenzyme A→acetyl-CoA + propanoyl-CoA (EC 2.3.1.16)
AZOBR_RS20220 -1.3
AZOBR_RS28180 -0.4
AZOBR_RS30610 +0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information