MetaCyc Pathway: L-isoleucine biosynthesis I (from threonine) in Pseudomonas fluorescens FW300-N2E2

Add experiment(s):


Reactions and Genes D-Xylose (C)
remove
Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
Pf6N2E2_1562 -0.2
Pf6N2E2_3938 +2.6
Pf6N2E2_802 -0.2
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
Pf6N2E2_1562 -0.2
Pf6N2E2_1819 +0.9
Pf6N2E2_2208 -0.2
Pf6N2E2_3938 +2.6
Pf6N2E2_5896 +0.2
Pf6N2E2_802 -0.2
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
Pf6N2E2_1562 -0.2
Pf6N2E2_1819 +0.9
Pf6N2E2_2208 -0.2
Pf6N2E2_3938 +2.6
Pf6N2E2_4360 +0.6
Pf6N2E2_5896 +0.2
Pf6N2E2_802 -0.2
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
Pf6N2E2_5175 -5.6
Pf6N2E2_5176 -5.4
Pf6N2E2_5475 -0.1
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
Pf6N2E2_5177 -4.5
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
Pf6N2E2_1668 -3.0
Pf6N2E2_4564 -4.4
Pf6N2E2_609 -0.1
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
Pf6N2E2_477 +0.0

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information