MetaCyc Pathway: 2,4-dinitrotoluene degradation in Shewanella sp. ANA-3

Reactions and Genes	L-Isoleucine (C)
2,4-dinitrotoluene + NADH + dioxygen→4-methyl-5-nitrocatechol + NAD+ + nitrite (EC 1.14.12.24)
No genes
4-methyl-5-nitrocatechol + NAD(P)H + dioxygen→2-hydroxy-5-methylquinone + NAD(P)+ + nitrite + H+ + H2O (EC 1.14.13.210)
No genes
2-hydroxy-5-methylquinone + NADH + 2 H+→2,4,5-trihydroxytoluene + NAD+
No genes
2,4,5-trihydroxytoluene + dioxygen→2,4-dihydroxy-5-methyl-6-oxohexa-2,4-dienoate + H+
No genes
2,4-dihydroxy-5-methyl-6-oxohexa-2,4-dienoate→4-hydroxy-2-keto-5-methyl-6-oxo-3-hexenoate + H+
No genes
4-hydroxy-2-keto-5-methyl-6-oxo-3-hexenoate + H2O→methylmalonate semialdehyde + pyruvate
No genes
Methylmalonate-semialdehyde dehydrogenase (acylating): methylmalonate semialdehyde + coenzyme A + NAD+ + H2O→propanoyl-CoA + hydrogen carbonate + NADH + H+ (EC 1.2.1.27)
Shewana3_2770	-5.0
Shewana3_3107	+0.0

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information