MetaCyc Pathway: dTMP de novo biosynthesis (mitochondrial) in Pseudomonas stutzeri RCH2

Reactions and Genes	butyrate (C)
Dihydrofolate reductase (in reverse): a 7,8-dihydrofolate + NADPH + H+→a tetrahydrofolate + NADP+ (EC 1.5.1.3)
Psest_0229	N.D.
Psest_3204	-2.0
Glycine hydroxymethyltransferase: a tetrahydrofolate + L-serine→a 5,10-methylenetetrahydrofolate + glycine + H2O (EC 2.1.2.1)
Psest_3404	N.D.
Thymidylate synthase: a 5,10-methylenetetrahydrofolate + dUMP→a 7,8-dihydrofolate + dTMP (EC 2.1.1.45)
Psest_3858	N.D.

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information