MetaCyc Pathway: cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) in Pseudomonas stutzeri RCH2

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Reactions and Genes nitrite (N)
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Uroporphyrinogen-III C-methyltransferase:
uroporphyrinogen-III + S-adenosyl-L-methionine→precorrin-1 + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.107)
Psest_1925 -2.0
Psest_2037 -2.3
Psest_3746 N.D.
Psest_0788 +0.5
Psest_0810 -0.1
Uroporphyrinogen-III C-methyltransferase:
precorrin-1 + S-adenosyl-L-methionine→precorrin-2 + S-adenosyl-L-homocysteine (EC 2.1.1.107)
Psest_1925 -2.0
Psest_2037 -2.3
Psest_3746 N.D.
Psest_0788 +0.5
Psest_0810 -0.1
Precorrin-2 dehydrogenase:
precorrin-2 + NAD+→sirohydrochlorin + NADH + 2 H+ (EC 1.3.1.76)
Psest_1925 -2.0
Psest_2037 -2.3
Psest_0810 -0.1
Sirohydrochlorin cobaltochelatase:
sirohydrochlorin + Co2+→cobalt-sirohydrochlorin + 2 H+ (EC 4.99.1.3)
No genes
Cobalt-factor II C(20)-methyltransferase:
cobalt-sirohydrochlorin + S-adenosyl-L-methionine→cobalt-factor III + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.151)
No genes
cobalt-factor III + a reduced two electron carrier→cobalt-precorrin-3 + an oxidized electron carrier (EC 2.1.1.272)
No genes
cobalt-precorrin-3 + S-adenosyl-L-methionine→cobalt-precorrin-4 + S-adenosyl-L-homocysteine (EC 2.1.1.272)
No genes
cobalt-precorrin-4 + S-adenosyl-L-methionine→cobalt-precorrin-5A + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.271)
No genes
cobalt-precorrin-5A + H2O→cobalt-precorrin-5B + acetaldehyde + H+ (EC 3.7.1.12)
No genes
cobalt-precorrin-5B + S-adenosyl-L-methionine→cobalt-precorrin-6A + S-adenosyl-L-homocysteine (EC 2.1.1.195)
No genes
cobalt-precorrin-6A + NADH→cobalt-precorrin-6B + NAD+ (EC 1.3.1.106)
No genes
cobalt-precorrin-6B + H+ + S-adenosyl-L-methionine→cobalt-precorrin-7 + S-adenosyl-L-homocysteine + CO2 (EC 2.1.1.196)
No genes
cobalt-precorrin-7 + S-adenosyl-L-methionine→cobalt-precorrin-8 + S-adenosyl-L-homocysteine (EC 2.1.1.289)
No genes
cobalt-precorrin-8→cobyrinate + H+ (EC 5.4.99.60)
No genes
cobyrinate + 2 ATP + 2 L-glutamine + 2 H2O→cob(II)yrinate a,c-diamide + 2 ADP + 2 L-glutamate + 2 H+ + 2 phosphate (EC 6.3.5.11)
Psest_3013 -0.6

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information