MetaCyc Pathway: tRNA-uridine 2-thiolation (mammalian mitochondria) in Pseudomonas stutzeri RCH2

Reactions and Genes	L-Alanine (N)
a uridine in tRNA + an oxidized electron carrier + GTP + a 5,10-methylenetetrahydrofolate + taurine + H2O→a 5-taurinomethyluridine in tRNA + a 7,8-dihydrofolate + a reduced two electron carrier + GDP + 2 H+ + phosphate
No genes
Cysteine desulfurase: L-cysteine + an [L-cysteine desulfurase]-L-cysteine→an [L-cysteine desulfurase]-S-sulfanyl-L-cysteine + L-alanine (EC 2.8.1.7)
Psest_1251	N.D.
Psest_1464	-1.4
Psest_1699	-0.3
an [L-cysteine desulfurase]-S-sulfanyl-L-cysteine + a [3-mercaptopyruvate sulfurtransferase]-L-cysteine→a [3-mercaptopyruvate sulfurtransferase]-S-sulfanyl-L-cysteine + an [L-cysteine desulfurase]-L-cysteine
No genes
a [3-mercaptopyruvate sulfurtransferase]-S-sulfanyl-L-cysteine + a 5-taurinomethyluridine in tRNA + ATP + a reduced two electron carrier→a 5-taurinomethyl-2-thiouridine in tRNA + AMP + an oxidized electron carrier + diphosphate + H+ + a [3-mercaptopyruvate sulfurtransferase]-L-cysteine (EC 2.8.1.14)
No genes

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information