MetaCyc Pathway: methylgallate degradation in Klebsiella michiganensis M5al

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Reactions and Genes Parafilmed volatile agar plate with no fungus
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3-O-methylgallate + dioxygen→2-pyrone-4,6-dicarboxylate + methanol + H+
No genes
2-pyrone-4,6-dicarboxylate lactonase:
2-pyrone-4,6-dicarboxylate + H2O→(1E)-4-oxobut-1-ene-1,2,4-tricarboxylate + H+
(EC 3.1.1.57)
No genes
(1E)-4-oxobut-1-ene-1,2,4-tricarboxylate→(1E,3E)-4-hydroxybuta-1,3-diene-1,2,4-tricarboxylate (spontaneous) (EC 5.3.2.8)
BWI76_RS04370 +0.1
BWI76_RS14180 -0.1
4-oxalmesaconate hydratase (in reverse):
(1E,3E)-4-hydroxybuta-1,3-diene-1,2,4-tricarboxylate + H2O→2-hydroxy-4-oxobutane-1,2,4-tricarboxylate
(EC 4.2.1.83)
BWI76_RS14190 +0.2
4-hydroxy-4-methyl-2-oxoglutarate aldolase:
2-hydroxy-4-oxobutane-1,2,4-tricarboxylate→oxaloacetate + pyruvate
(EC 4.1.3.17)
BWI76_RS14185 -0.1
Malate dehydrogenase (oxaloacetate-decarboxylating):
oxaloacetate + H+→pyruvate + CO2
(EC 1.1.1.38; 1.1.1.40; 1.1.1.83; 4.1.1.112)
BWI76_RS04415 N.D.
BWI76_RS04420 -0.6
BWI76_RS04425 -0.3
BWI76_RS10680 +0.3
BWI76_RS13830 +0.5
BWI76_RS18095 N.D.
BWI76_RS20830 -0.1
BWI76_RS25380 +2.2
BWI76_RS25385 +0.6
BWI76_RS25390 +0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information