MetaCyc Pathway: (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) in Pseudomonas fluorescens SBW25-INTG

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Reactions and Genes L-Carnitine (C)
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Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
PFLU_RS03225 +0.0
PFLU_RS06735 -0.5
PFLU_RS07655 -0.2
PFLU_RS10575 -1.6
PFLU_RS14765 -0.7
PFLU_RS16065 +0.1
PFLU_RS17190 -0.3
PFLU_RS21180 +0.3
PFLU_RS22845 -0.8
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
PFLU_RS07650 -2.2
PFLU_RS11090 +0.3
PFLU_RS22840 +0.4
Acetoacetyl-CoA reductase (in reverse):
acetoacetyl-CoA + NADPH + H+→(3R)-3-hydroxybutanoyl-CoA + NADP+ (EC 1.1.1.36)
No genes
(S)-3-hydroxybutanoyl-CoA + H2O→(S)-3-hydroxybutanoate + coenzyme A + H+
PFLU_RS26405 -0.1
(3R)-3-hydroxybutanoyl-CoA + H2O→(R)-3-hydroxybutanoate + coenzyme A + H+
PFLU_RS26405 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information