MetaCyc Pathway: cis-vaccenate biosynthesis in Pseudomonas fluorescens SBW25-INTG

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Reactions and Genes D-(+)-Galactosamine (C)
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Beta-ketoacyl-acyl-carrier-protein synthase II:
a palmitoleoyl-[acp] + a malonyl-[acp] + H+→an (11Z)-3-oxooctadec-11-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
PFLU_RS02075 +0.1
PFLU_RS15160 +0.4
PFLU_RS15590 +0.1
PFLU_RS23045 -0.1
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
an (11Z)-3-oxooctadec-11-enoyl-[acp] + NADPH + H+→a (3R,11Z)-3-hydroxyoctadec-11-enoyl-[acp] + NADP+ (EC 1.1.1.100)
PFLU_RS02080 -0.0
PFLU_RS03220 -0.1
PFLU_RS05335 -0.1
PFLU_RS05340 +0.7
PFLU_RS09615 +0.5
PFLU_RS09635 -0.6
PFLU_RS09805 +0.0
PFLU_RS09810 -0.0
PFLU_RS12555 -0.1
PFLU_RS14865 +0.3
PFLU_RS14960 -0.6
PFLU_RS15050 -0.3
PFLU_RS15565 -0.1
PFLU_RS15990 +0.3
PFLU_RS16275 -0.2
PFLU_RS17200 -0.1
PFLU_RS18190 +0.1
PFLU_RS19495 -0.4
PFLU_RS22760 +0.0
PFLU_RS22960 -2.6
PFLU_RS23055 N.D.
PFLU_RS30525 -0.4
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,11Z)-3-hydroxyoctadec-11-enoyl-[acp]→a (2E,11Z)-octadeca-2,11-dienoyl-[acp] + H2O (EC 4.2.1.59)
PFLU_RS06330 N.D.
PFLU_RS09035 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,11Z)-octadeca-2,11-dienoyl-[acp] + NADH + H+→a cis-vaccenoyl-[acp] + NAD+ (EC 1.3.1.9)
PFLU_RS14490 +0.0
Oleoyl-[acyl-carrier-protein] hydrolase:
a cis-vaccenoyl-[acp] + H2O→cis-vaccenate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information