MetaCyc Pathway: 2-oxoisovalerate decarboxylation to isobutanoyl-CoA in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes Ying_AminoAcid20 rep C; time point 3
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2-oxoisovalerate dehydrogenase (acylating):
a [BCAA dehydrogenase E2 protein] N6-lipoyl-L-lysine + 3-methyl-2-oxobutanoate + H+→an [apo BCAA dehydrogenase E2 protein] N6-S-[2-methylpropanoyl]dihydrolipoyl-L-lysine + CO2 (EC 1.2.1.25; 1.2.4.4)
Pf6N2E2_480 -0.6
Pf6N2E2_481 -1.2
2-oxoisovalerate dehydrogenase (acylating) (in reverse):
an [apo BCAA dehydrogenase E2 protein] N6-S-[2-methylpropanoyl]dihydrolipoyl-L-lysine + coenzyme A→an [apo BCAA dehydrogenase E2 protein] N6-dihydrolipoyl-L-lysine + isobutanoyl-CoA (EC 1.2.1.25; 2.3.1.168)
Pf6N2E2_479 -1.9
2-oxoisovalerate dehydrogenase (acylating):
an [apo BCAA dehydrogenase E2 protein] N6-dihydrolipoyl-L-lysine + NAD+→a [BCAA dehydrogenase E2 protein] N6-lipoyl-L-lysine + NADH + H+ (EC 1.2.1.25; 1.8.1.4)
Pf6N2E2_4251 +0.0
Pf6N2E2_478 -0.2
Pf6N2E2_5857 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information