MetaCyc Pathway: 3-phenylpropanoate degradation in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes Ying_AminoAcid20 rep A; time point 2
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3-phenylpropanoate + ATP + coenzyme A→3-phenylpropanoyl-CoA + AMP + diphosphate
No genes
3-phenylpropanoyl-CoA + an oxidized electron carrier→(E)-cinnamoyl-CoA + a reduced two electron carrier
No genes
Enoyl-CoA hydratase (in reverse):
(E)-cinnamoyl-CoA + H2O→(S)-3-hydroxy-3-phenylpropanoyl-CoA
(EC 4.2.1.17)
Pf6N2E2_1013 -0.1
Pf6N2E2_1046 +0.0
Pf6N2E2_1147 -2.3
Pf6N2E2_1149 -0.1
Pf6N2E2_1269 +0.1
Pf6N2E2_1833 -0.1
Pf6N2E2_1834 -1.2
Pf6N2E2_1835 +0.0
Pf6N2E2_1840 +0.8
Pf6N2E2_1850 -0.2
Pf6N2E2_1922 +0.1
Pf6N2E2_1934 -0.0
Pf6N2E2_1935 +0.9
Pf6N2E2_2290 N.D.
Pf6N2E2_2773 +0.1
Pf6N2E2_3894 +0.1
Pf6N2E2_5493 -0.2
Pf6N2E2_6000 +0.0
Pf6N2E2_6004 N.D.
Pf6N2E2_742 +0.2
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxy-3-phenylpropanoyl-CoA + NAD+→3-oxo-3-phenylpropanoyl-CoA + NADH + H+
(EC 1.1.1.35)
Pf6N2E2_1833 -0.1
Pf6N2E2_1850 -0.2
Pf6N2E2_1922 +0.1
Pf6N2E2_2290 N.D.
Pf6N2E2_3894 +0.1
Pf6N2E2_4690 -0.4
Pf6N2E2_6000 +0.0
Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-3-phenylpropanoyl-CoA + coenzyme A→benzoyl-CoA + acetyl-CoA
(EC 2.3.1.16)
Pf6N2E2_1145 N.D.
Pf6N2E2_1675 +0.0
Pf6N2E2_1836 -0.2
Pf6N2E2_1851 +0.0
Pf6N2E2_2113 -0.6
Pf6N2E2_2289 N.D.
Pf6N2E2_2539 +0.1
Pf6N2E2_2835 +0.6
Pf6N2E2_3492 -0.2
Pf6N2E2_5999 -0.8
Benzoyl-CoA 3-monooxygenase:
benzoyl-CoA + NADPH + dioxygen + H+→3-hydroxybenzoyl-CoA + NADP+ + H2O
(EC 1.14.13.58)
No genes
3-hydroxybenzoyl-CoA + a reduced two electron carrier + dioxygen→gentisate + an oxidized electron carrier + coenzyme A + H+
No genes
Gentisate 1,2-dioxygenase:
gentisate + dioxygen→3-maleylpyruvate + H+
(EC 1.13.11.4)
No genes
Maleylpyruvate isomerase:
3-maleylpyruvate→3-fumarylpyruvate
(EC 5.2.1.4)
Pf6N2E2_5292 -1.4
3-fumarylpyruvate + H2O→fumarate + pyruvate + H+ (EC 3.7.1.20)
Pf6N2E2_6018 -0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information