MetaCyc Pathway: polyhydroxybutanoate biosynthesis in Pseudomonas fluorescens SBW25-INTG

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Reactions and Genes L-Isoleucine (C) 10 mM
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Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
PFLU_RS03225 -0.1
PFLU_RS06735 +0.2
PFLU_RS07655 +0.6
PFLU_RS10575 -0.1
PFLU_RS14765 -4.4
PFLU_RS16065 +0.1
PFLU_RS17190 -0.2
PFLU_RS21180 -0.2
PFLU_RS22845 -0.1
Acetoacetyl-CoA reductase (in reverse):
acetoacetyl-CoA + NADPH + H+→(3R)-3-hydroxybutanoyl-CoA + NADP+ (EC 1.1.1.36)
No genes
(3R)-3-hydroxybutanoyl-CoA + poly-[(R)-3-hydroxybutanoate]→poly-[(R)-3-hydroxybutanoate] + coenzyme A (EC 2.3.1.304)
PFLU_RS06000 -0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information