MetaCyc Pathway: propanoate fermentation to 2-methylbutanoate in Pseudomonas fluorescens SBW25-INTG

Add experiment(s):


Reactions and Genes propionate 20 mM (C)
remove
Propionate CoA-transferase:
propanoate + acetyl-CoA→acetate + propanoyl-CoA (EC 2.8.3.1)
No genes
Acetyl-CoA C-acyltransferase:
propanoyl-CoA + acetyl-CoA→2-methylacetoacetyl-CoA + coenzyme A (EC 2.3.1.16)
PFLU_RS03225 -0.0
PFLU_RS06735 -0.8
PFLU_RS07655 -1.3
PFLU_RS10575 -0.2
PFLU_RS14765 +0.1
PFLU_RS16065 -0.3
PFLU_RS17190 +0.1
PFLU_RS21180 -0.2
PFLU_RS22845 -0.3
3-hydroxy-2-methylbutyryl-CoA dehydrogenase (in reverse):
2-methylacetoacetyl-CoA + NADH + H+→(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+ (EC 1.1.1.178)
PFLU_RS07650 -2.4
PFLU_RS11090 +0.2
PFLU_RS14760 +0.2
PFLU_RS22840 +0.4
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA→(E)-2-methylcrotonoyl-CoA + H2O (EC 4.2.1.150)
PFLU_RS07105 -1.9
PFLU_RS07650 -2.4
PFLU_RS08485 -1.1
PFLU_RS10835 +0.1
PFLU_RS12025 +0.3
PFLU_RS22840 +0.4
PFLU_RS24260 +0.2
(E)-2-methylcrotonoyl-CoA + a reduced electron-transfer flavoprotein→(2S)-2-methylbutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+ (EC 1.3.8.5)
No genes
(2S)-2-methylbutanoyl-CoA + H2O→2-methylbutanoate + coenzyme A + H+
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information