MetaCyc Pathway: even iso-branched-chain fatty acid biosynthesis in Pseudomonas fluorescens SBW25-INTG

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Reactions and Genes Arabitol 10 mM (C)
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Branched-chain-amino-acid transaminase:
L-valine + 2-oxoglutarate→3-methyl-2-oxobutanoate + L-glutamate (EC 2.6.1.42)
PFLU_RS19375 -0.1
2-oxoisovalerate dehydrogenase (acylating):
3-methyl-2-oxobutanoate + coenzyme A + NAD+→isobutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
isobutanoyl-CoA + a malonyl-[acp] + H+→a 4-methyl-3-oxopentanoyl-[acp] + CO2 + coenzyme A (EC 2.3.1.300)
No genes
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 4-methyl-3-oxopentanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-4-methylpentanoyl-[acp] + NADP+ (EC 1.1.1.100)
PFLU_RS02080 +0.3
PFLU_RS03220 +0.0
PFLU_RS05335 -0.1
PFLU_RS05340 +0.1
PFLU_RS09615 -0.3
PFLU_RS09635 +0.0
PFLU_RS09805 -0.1
PFLU_RS09810 -0.1
PFLU_RS12555 +0.3
PFLU_RS14865 -0.1
PFLU_RS14960 -0.1
PFLU_RS15050 -0.0
PFLU_RS15565 +0.3
PFLU_RS15990 -0.0
PFLU_RS16275 -0.2
PFLU_RS17200 -0.0
PFLU_RS18190 -0.1
PFLU_RS19495 -4.1
PFLU_RS22760 -0.1
PFLU_RS22960 +0.2
PFLU_RS23055 N.D.
PFLU_RS30525 +0.3
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-4-methylpentanoyl-[acp]→a (2E)-4-methylpent-2-enoyl-[acp] + H2O (EC 4.2.1.59)
PFLU_RS06330 N.D.
PFLU_RS09035 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-4-methylpent-2-enoyl-[acp] + NADH + H+→a 5-methylhexanoyl-[acp] + NAD+ (EC 1.3.1.9)
PFLU_RS14490 -0.1
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-4-methylpent-2-enoyl-[acp] + NADPH + H+→a 5-methylhexanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a 5-methylhexanoyl-[acp] + a malonyl-[acp] + H+→a 6-methyl-3-oxoheptanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
PFLU_RS02075 +0.0
PFLU_RS15160 -0.3
PFLU_RS15590 +0.7
PFLU_RS23045 -0.5
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 6-methyl-3-oxoheptanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-6-methylheptanoyl-[acp] + NADP+ (EC 1.1.1.100)
PFLU_RS02080 +0.3
PFLU_RS03220 +0.0
PFLU_RS05335 -0.1
PFLU_RS05340 +0.1
PFLU_RS09615 -0.3
PFLU_RS09635 +0.0
PFLU_RS09805 -0.1
PFLU_RS09810 -0.1
PFLU_RS12555 +0.3
PFLU_RS14865 -0.1
PFLU_RS14960 -0.1
PFLU_RS15050 -0.0
PFLU_RS15565 +0.3
PFLU_RS15990 -0.0
PFLU_RS16275 -0.2
PFLU_RS17200 -0.0
PFLU_RS18190 -0.1
PFLU_RS19495 -4.1
PFLU_RS22760 -0.1
PFLU_RS22960 +0.2
PFLU_RS23055 N.D.
PFLU_RS30525 +0.3
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-6-methylheptanoyl-[acp]→a (2E)-6-methylhept-2-enoyl-[acp] + H2O (EC 4.2.1.59)
PFLU_RS06330 N.D.
PFLU_RS09035 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-6-methylhept-2-enoyl-[acp] + NADH + H+→a 6-methylheptanoyl-[acp] + NAD+ (EC 1.3.1.9)
PFLU_RS14490 -0.1
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-6-methylhept-2-enoyl-[acp] + NADPH + H+→a 6-methylheptanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a 6-methylheptanoyl-[acp] + a malonyl-[acp] + H+→an 8-methyl-3-oxononanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
PFLU_RS02075 +0.0
PFLU_RS15160 -0.3
PFLU_RS15590 +0.7
PFLU_RS23045 -0.5
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
an 8-methyl-3-oxononanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-8-methylnonanoyl-[acp] + NADP+ (EC 1.1.1.100)
PFLU_RS02080 +0.3
PFLU_RS03220 +0.0
PFLU_RS05335 -0.1
PFLU_RS05340 +0.1
PFLU_RS09615 -0.3
PFLU_RS09635 +0.0
PFLU_RS09805 -0.1
PFLU_RS09810 -0.1
PFLU_RS12555 +0.3
PFLU_RS14865 -0.1
PFLU_RS14960 -0.1
PFLU_RS15050 -0.0
PFLU_RS15565 +0.3
PFLU_RS15990 -0.0
PFLU_RS16275 -0.2
PFLU_RS17200 -0.0
PFLU_RS18190 -0.1
PFLU_RS19495 -4.1
PFLU_RS22760 -0.1
PFLU_RS22960 +0.2
PFLU_RS23055 N.D.
PFLU_RS30525 +0.3
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-8-methylnonanoyl-[acp]→a (2E)-8-methylnon-2-enoyl-[acp] + H2O (EC 4.2.1.59)
PFLU_RS06330 N.D.
PFLU_RS09035 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-8-methylnon-2-enoyl-[acp] + NADH + H+→an 8-methylnonanoyl-[acyl-carrier protein] + NAD+ (EC 1.3.1.9)
PFLU_RS14490 -0.1
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-8-methylnon-2-enoyl-[acp] + NADPH + H+→an 8-methylnonanoyl-[acyl-carrier protein] + NADP+ (EC 1.3.1.10; 1.3.1.104)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
an 8-methylnonanoyl-[acyl-carrier protein] + a malonyl-[acp] + H+→a 1o-methyl-3-oxoundecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
PFLU_RS02075 +0.0
PFLU_RS15160 -0.3
PFLU_RS15590 +0.7
PFLU_RS23045 -0.5
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 1o-methyl-3-oxoundecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-10-methylundecanoyl-[acp] + NADP+ (EC 1.1.1.100)
PFLU_RS02080 +0.3
PFLU_RS03220 +0.0
PFLU_RS05335 -0.1
PFLU_RS05340 +0.1
PFLU_RS09615 -0.3
PFLU_RS09635 +0.0
PFLU_RS09805 -0.1
PFLU_RS09810 -0.1
PFLU_RS12555 +0.3
PFLU_RS14865 -0.1
PFLU_RS14960 -0.1
PFLU_RS15050 -0.0
PFLU_RS15565 +0.3
PFLU_RS15990 -0.0
PFLU_RS16275 -0.2
PFLU_RS17200 -0.0
PFLU_RS18190 -0.1
PFLU_RS19495 -4.1
PFLU_RS22760 -0.1
PFLU_RS22960 +0.2
PFLU_RS23055 N.D.
PFLU_RS30525 +0.3
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-10-methylundecanoyl-[acp]→a (2E)-10-methylundec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
PFLU_RS06330 N.D.
PFLU_RS09035 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-10-methylundec-2-enoyl-[acp] + NADH + H+→an 10-methylundecanoyl-[acp] + NAD+ (EC 1.3.1.9)
PFLU_RS14490 -0.1
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-10-methylundec-2-enoyl-[acp] + NADPH + H+→an 10-methylundecanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
an 10-methylundecanoyl-[acp] + a malonyl-[acp] + H+→a 12-methyl-3-oxotridecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
PFLU_RS02075 +0.0
PFLU_RS15160 -0.3
PFLU_RS15590 +0.7
PFLU_RS23045 -0.5
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 12-methyl-3-oxotridecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-12-methyltridecanoyl-[acp] + NADP+ (EC 1.1.1.100)
PFLU_RS02080 +0.3
PFLU_RS03220 +0.0
PFLU_RS05335 -0.1
PFLU_RS05340 +0.1
PFLU_RS09615 -0.3
PFLU_RS09635 +0.0
PFLU_RS09805 -0.1
PFLU_RS09810 -0.1
PFLU_RS12555 +0.3
PFLU_RS14865 -0.1
PFLU_RS14960 -0.1
PFLU_RS15050 -0.0
PFLU_RS15565 +0.3
PFLU_RS15990 -0.0
PFLU_RS16275 -0.2
PFLU_RS17200 -0.0
PFLU_RS18190 -0.1
PFLU_RS19495 -4.1
PFLU_RS22760 -0.1
PFLU_RS22960 +0.2
PFLU_RS23055 N.D.
PFLU_RS30525 +0.3
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-12-methyltridecanoyl-[acp]→a (2E)-12-methyltridec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
PFLU_RS06330 N.D.
PFLU_RS09035 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-12-methyltridec-2-enoyl-[acp] + NADH + H+→a 12-methyltridecanoyl-[acp] + NAD+ (EC 1.3.1.9)
PFLU_RS14490 -0.1
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-12-methyltridec-2-enoyl-[acp] + NADPH + H+→a 12-methyltridecanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a 12-methyltridecanoyl-[acp] + a malonyl-[acp] + H+→a 14-methyl-3-oxopentadecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
PFLU_RS02075 +0.0
PFLU_RS15160 -0.3
PFLU_RS15590 +0.7
PFLU_RS23045 -0.5
Oleoyl-[acyl-carrier-protein] hydrolase:
a 12-methyltridecanoyl-[acp] + H2O→12-methyltridecanoate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
No genes
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 14-methyl-3-oxopentadecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-14-methylpentadecanoyl-[acp] + NADP+ (EC 1.1.1.100)
PFLU_RS02080 +0.3
PFLU_RS03220 +0.0
PFLU_RS05335 -0.1
PFLU_RS05340 +0.1
PFLU_RS09615 -0.3
PFLU_RS09635 +0.0
PFLU_RS09805 -0.1
PFLU_RS09810 -0.1
PFLU_RS12555 +0.3
PFLU_RS14865 -0.1
PFLU_RS14960 -0.1
PFLU_RS15050 -0.0
PFLU_RS15565 +0.3
PFLU_RS15990 -0.0
PFLU_RS16275 -0.2
PFLU_RS17200 -0.0
PFLU_RS18190 -0.1
PFLU_RS19495 -4.1
PFLU_RS22760 -0.1
PFLU_RS22960 +0.2
PFLU_RS23055 N.D.
PFLU_RS30525 +0.3
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-14-methylpentadecanoyl-[acp]→a (2E)-14-methylpentadec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
PFLU_RS06330 N.D.
PFLU_RS09035 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-14-methylpentadec-2-enoyl-[acp] + NADH + H+→a 14-methylpentadecanoyl-[acp] + NAD+ (EC 1.3.1.9)
PFLU_RS14490 -0.1
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-14-methylpentadec-2-enoyl-[acp] + NADPH + H+→a 14-methylpentadecanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.39)
No genes
Oleoyl-[acyl-carrier-protein] hydrolase:
a 14-methylpentadecanoyl-[acp] + H2O→14-methylpentadecanoate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information