MetaCyc Pathway: stearate biosynthesis II (bacteria and plants) in Pseudomonas fluorescens SBW25-INTG

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Reactions and Genes Arabitol 10 mM (C)
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Beta-ketoacyl-acyl-carrier-protein synthase I:
a palmitoyl-[acp] + a malonyl-[acp] + H+→a 3-oxooctadecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.41)
PFLU_RS02075 +0.0
PFLU_RS02090 -0.0
PFLU_RS09030 N.D.
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxooctadecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxyoctadecanoyl-[acp] + NADP+ (EC 1.1.1.100)
PFLU_RS02080 +0.3
PFLU_RS03220 +0.0
PFLU_RS05335 -0.1
PFLU_RS05340 +0.1
PFLU_RS09615 -0.3
PFLU_RS09635 +0.0
PFLU_RS09805 -0.1
PFLU_RS09810 -0.1
PFLU_RS12555 +0.3
PFLU_RS14865 -0.1
PFLU_RS14960 -0.1
PFLU_RS15050 -0.0
PFLU_RS15565 +0.3
PFLU_RS15990 -0.0
PFLU_RS16275 -0.2
PFLU_RS17200 -0.0
PFLU_RS18190 -0.1
PFLU_RS19495 -4.1
PFLU_RS22760 -0.1
PFLU_RS22960 +0.2
PFLU_RS23055 N.D.
PFLU_RS30525 +0.3
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyoctadecanoyl-[acp]→a (2E)-octadec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
PFLU_RS06330 N.D.
PFLU_RS09035 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-octadec-2-enoyl-[acp] + NADH + H+→a stearoyl-[acp] + NAD+ (EC 1.3.1.9)
PFLU_RS14490 -0.1
Oleoyl-[acyl-carrier-protein] hydrolase:
a stearoyl-[acp] + H2O→stearate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
No genes
Long-chain-fatty-acid--CoA ligase:
stearate + ATP + coenzyme A→stearoyl-CoA + AMP + diphosphate (EC 6.2.1.3)
PFLU_RS17185 -0.2
PFLU_RS21255 +0.0
PFLU_RS23680 -0.4
PFLU_RS23685 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information