MetaCyc Pathway: odd iso-branched-chain fatty acid biosynthesis in Burkholderia phytofirmans PsJN

Add experiment(s):


Reactions and Genes Parafilmed volatile agar plate with Trichoderma atroviridae IMI
remove
Branched-chain-amino-acid transaminase:
L-leucine + 2-oxoglutarate→4-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42; 2.6.1.6)
BPHYT_RS16285 +0.3
2-oxoisovalerate dehydrogenase (acylating):
4-methyl-2-oxopentanoate + coenzyme A + NAD+→3-methylbutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
3-methylbutanoyl-CoA + a malonyl-[acp] + H+→a 5-methyl-3-oxohexanoyl-[acp] + CO2 + coenzyme A (EC 2.3.1.300)
No genes
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 5-methyl-3-oxohexanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-5-methylhexanoyl-[acp] + NADP+ (EC 1.1.1.100)
BPHYT_RS01050 +0.3
BPHYT_RS04770 -0.2
BPHYT_RS05365 +0.4
BPHYT_RS05385 -0.1
BPHYT_RS06420 N.D.
BPHYT_RS07065 +0.5
BPHYT_RS08285 N.D.
BPHYT_RS08365 +0.4
BPHYT_RS08890 -0.0
BPHYT_RS10170 +0.1
BPHYT_RS10200 N.D.
BPHYT_RS10595 +0.2
BPHYT_RS10630 +0.1
BPHYT_RS10735 N.D.
BPHYT_RS11845 N.D.
BPHYT_RS13055 +2.8
BPHYT_RS14425 N.D.
BPHYT_RS15135 +0.2
BPHYT_RS15890 -0.2
BPHYT_RS16040 N.D.
BPHYT_RS16940 +0.6
BPHYT_RS20510 N.D.
BPHYT_RS20625 -0.3
BPHYT_RS20775 -0.2
BPHYT_RS21600 +0.3
BPHYT_RS22135 +2.5
BPHYT_RS25030 -0.3
BPHYT_RS25415 N.D.
BPHYT_RS25655 +0.8
BPHYT_RS26470 -1.1
BPHYT_RS28250 N.D.
BPHYT_RS30440 +0.0
BPHYT_RS30465 -1.5
BPHYT_RS30795 +0.0
BPHYT_RS31385 -0.5
BPHYT_RS31405 N.D.
BPHYT_RS31610 -0.4
BPHYT_RS31900 +0.1
BPHYT_RS33140 N.D.
BPHYT_RS33705 +0.0
BPHYT_RS34945 N.D.
BPHYT_RS34965 N.D.
BPHYT_RS35505 -0.2
BPHYT_RS35675 +0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-5-methylhexanoyl-[acp]→a (2E)-5-methylhex-2-enoyl-[acp] + H2O (EC 4.2.1.59)
BPHYT_RS12085 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-5-methylhex-2-enoyl-[acp] + NADH + H+→a 5-methylhexanoyl-[acp] + NAD+ (EC 1.3.1.9)
BPHYT_RS01880 -0.1
BPHYT_RS12390 +0.3
BPHYT_RS20615 -0.4
BPHYT_RS30015 N.D.
BPHYT_RS33380 +0.1
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-5-methylhex-2-enoyl-[acp] + NADPH + H+→a 5-methylhexanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104)
BPHYT_RS32935 +0.0
Beta-ketoacyl-acyl-carrier-protein synthase II:
a 5-methylhexanoyl-[acp] + a malonyl-[acp] + H+→a 7-methyl-3-oxooctanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
BPHYT_RS14415 N.D.
BPHYT_RS15130 +0.0
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 7-methyl-3-oxooctanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-7-methyloctanoyl-[acp] + NADP+ (EC 1.1.1.100)
BPHYT_RS01050 +0.3
BPHYT_RS04770 -0.2
BPHYT_RS05365 +0.4
BPHYT_RS05385 -0.1
BPHYT_RS06420 N.D.
BPHYT_RS07065 +0.5
BPHYT_RS08285 N.D.
BPHYT_RS08365 +0.4
BPHYT_RS08890 -0.0
BPHYT_RS10170 +0.1
BPHYT_RS10200 N.D.
BPHYT_RS10595 +0.2
BPHYT_RS10630 +0.1
BPHYT_RS10735 N.D.
BPHYT_RS11845 N.D.
BPHYT_RS13055 +2.8
BPHYT_RS14425 N.D.
BPHYT_RS15135 +0.2
BPHYT_RS15890 -0.2
BPHYT_RS16040 N.D.
BPHYT_RS16940 +0.6
BPHYT_RS20510 N.D.
BPHYT_RS20625 -0.3
BPHYT_RS20775 -0.2
BPHYT_RS21600 +0.3
BPHYT_RS22135 +2.5
BPHYT_RS25030 -0.3
BPHYT_RS25415 N.D.
BPHYT_RS25655 +0.8
BPHYT_RS26470 -1.1
BPHYT_RS28250 N.D.
BPHYT_RS30440 +0.0
BPHYT_RS30465 -1.5
BPHYT_RS30795 +0.0
BPHYT_RS31385 -0.5
BPHYT_RS31405 N.D.
BPHYT_RS31610 -0.4
BPHYT_RS31900 +0.1
BPHYT_RS33140 N.D.
BPHYT_RS33705 +0.0
BPHYT_RS34945 N.D.
BPHYT_RS34965 N.D.
BPHYT_RS35505 -0.2
BPHYT_RS35675 +0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-7-methyloctanoyl-[acp]→a (2E)-7-methyloct-2-enoyl-[acp] + H2O (EC 4.2.1.59)
BPHYT_RS12085 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-7-methyloct-2-enoyl-[acp] + NADH + H+→a 7-methyloctanoyl-[acp] + NAD+ (EC 1.3.1.9)
BPHYT_RS01880 -0.1
BPHYT_RS12390 +0.3
BPHYT_RS20615 -0.4
BPHYT_RS30015 N.D.
BPHYT_RS33380 +0.1
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-7-methyloct-2-enoyl-[acp] + NADPH + H+→a 7-methyloctanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104)
BPHYT_RS32935 +0.0
Beta-ketoacyl-acyl-carrier-protein synthase II:
a 7-methyloctanoyl-[acp] + a malonyl-[acp] + H+→a 9-methyl-3-oxodecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
BPHYT_RS14415 N.D.
BPHYT_RS15130 +0.0
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 9-methyl-3-oxodecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-9-methyldecanoyl-[acp] + NADP+ (EC 1.1.1.100)
BPHYT_RS01050 +0.3
BPHYT_RS04770 -0.2
BPHYT_RS05365 +0.4
BPHYT_RS05385 -0.1
BPHYT_RS06420 N.D.
BPHYT_RS07065 +0.5
BPHYT_RS08285 N.D.
BPHYT_RS08365 +0.4
BPHYT_RS08890 -0.0
BPHYT_RS10170 +0.1
BPHYT_RS10200 N.D.
BPHYT_RS10595 +0.2
BPHYT_RS10630 +0.1
BPHYT_RS10735 N.D.
BPHYT_RS11845 N.D.
BPHYT_RS13055 +2.8
BPHYT_RS14425 N.D.
BPHYT_RS15135 +0.2
BPHYT_RS15890 -0.2
BPHYT_RS16040 N.D.
BPHYT_RS16940 +0.6
BPHYT_RS20510 N.D.
BPHYT_RS20625 -0.3
BPHYT_RS20775 -0.2
BPHYT_RS21600 +0.3
BPHYT_RS22135 +2.5
BPHYT_RS25030 -0.3
BPHYT_RS25415 N.D.
BPHYT_RS25655 +0.8
BPHYT_RS26470 -1.1
BPHYT_RS28250 N.D.
BPHYT_RS30440 +0.0
BPHYT_RS30465 -1.5
BPHYT_RS30795 +0.0
BPHYT_RS31385 -0.5
BPHYT_RS31405 N.D.
BPHYT_RS31610 -0.4
BPHYT_RS31900 +0.1
BPHYT_RS33140 N.D.
BPHYT_RS33705 +0.0
BPHYT_RS34945 N.D.
BPHYT_RS34965 N.D.
BPHYT_RS35505 -0.2
BPHYT_RS35675 +0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-9-methyldecanoyl-[acp]→a (2E)-9-methyldec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
BPHYT_RS12085 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-9-methyldec-2-enoyl-[acp] + NADH + H+→a 9-methyldecanoyl-[acyl-carrier protein] + NAD+ (EC 1.3.1.9)
BPHYT_RS01880 -0.1
BPHYT_RS12390 +0.3
BPHYT_RS20615 -0.4
BPHYT_RS30015 N.D.
BPHYT_RS33380 +0.1
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-9-methyldec-2-enoyl-[acp] + NADPH + H+→a 9-methyldecanoyl-[acyl-carrier protein] + NADP+ (EC 1.3.1.10; 1.3.1.104)
BPHYT_RS32935 +0.0
Beta-ketoacyl-acyl-carrier-protein synthase II:
a 9-methyldecanoyl-[acyl-carrier protein] + a malonyl-[acp] + H+→a 11-methyl-3-oxododecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
BPHYT_RS14415 N.D.
BPHYT_RS15130 +0.0
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 11-methyl-3-oxododecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-11-methyldodecanoyl-[acp] + NADP+ (EC 1.1.1.100)
BPHYT_RS01050 +0.3
BPHYT_RS04770 -0.2
BPHYT_RS05365 +0.4
BPHYT_RS05385 -0.1
BPHYT_RS06420 N.D.
BPHYT_RS07065 +0.5
BPHYT_RS08285 N.D.
BPHYT_RS08365 +0.4
BPHYT_RS08890 -0.0
BPHYT_RS10170 +0.1
BPHYT_RS10200 N.D.
BPHYT_RS10595 +0.2
BPHYT_RS10630 +0.1
BPHYT_RS10735 N.D.
BPHYT_RS11845 N.D.
BPHYT_RS13055 +2.8
BPHYT_RS14425 N.D.
BPHYT_RS15135 +0.2
BPHYT_RS15890 -0.2
BPHYT_RS16040 N.D.
BPHYT_RS16940 +0.6
BPHYT_RS20510 N.D.
BPHYT_RS20625 -0.3
BPHYT_RS20775 -0.2
BPHYT_RS21600 +0.3
BPHYT_RS22135 +2.5
BPHYT_RS25030 -0.3
BPHYT_RS25415 N.D.
BPHYT_RS25655 +0.8
BPHYT_RS26470 -1.1
BPHYT_RS28250 N.D.
BPHYT_RS30440 +0.0
BPHYT_RS30465 -1.5
BPHYT_RS30795 +0.0
BPHYT_RS31385 -0.5
BPHYT_RS31405 N.D.
BPHYT_RS31610 -0.4
BPHYT_RS31900 +0.1
BPHYT_RS33140 N.D.
BPHYT_RS33705 +0.0
BPHYT_RS34945 N.D.
BPHYT_RS34965 N.D.
BPHYT_RS35505 -0.2
BPHYT_RS35675 +0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-11-methyldodecanoyl-[acp]→a (2E)-11-methyldodec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
BPHYT_RS12085 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-11-methyldodec-2-enoyl-[acp] + NADH + H+→an 11-methyldodecanoyl-[acp] + NAD+ (EC 1.3.1.9)
BPHYT_RS01880 -0.1
BPHYT_RS12390 +0.3
BPHYT_RS20615 -0.4
BPHYT_RS30015 N.D.
BPHYT_RS33380 +0.1
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-11-methyldodec-2-enoyl-[acp] + NADPH + H+→an 11-methyldodecanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104)
BPHYT_RS32935 +0.0
Beta-ketoacyl-acyl-carrier-protein synthase II:
an 11-methyldodecanoyl-[acp] + a malonyl-[acp] + H+→a 13-methyl-3-oxotetradecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
BPHYT_RS14415 N.D.
BPHYT_RS15130 +0.0
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 13-methyl-3-oxotetradecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-13-methyltetradecanoyl-[acp] + NADP+ (EC 1.1.1.100)
BPHYT_RS01050 +0.3
BPHYT_RS04770 -0.2
BPHYT_RS05365 +0.4
BPHYT_RS05385 -0.1
BPHYT_RS06420 N.D.
BPHYT_RS07065 +0.5
BPHYT_RS08285 N.D.
BPHYT_RS08365 +0.4
BPHYT_RS08890 -0.0
BPHYT_RS10170 +0.1
BPHYT_RS10200 N.D.
BPHYT_RS10595 +0.2
BPHYT_RS10630 +0.1
BPHYT_RS10735 N.D.
BPHYT_RS11845 N.D.
BPHYT_RS13055 +2.8
BPHYT_RS14425 N.D.
BPHYT_RS15135 +0.2
BPHYT_RS15890 -0.2
BPHYT_RS16040 N.D.
BPHYT_RS16940 +0.6
BPHYT_RS20510 N.D.
BPHYT_RS20625 -0.3
BPHYT_RS20775 -0.2
BPHYT_RS21600 +0.3
BPHYT_RS22135 +2.5
BPHYT_RS25030 -0.3
BPHYT_RS25415 N.D.
BPHYT_RS25655 +0.8
BPHYT_RS26470 -1.1
BPHYT_RS28250 N.D.
BPHYT_RS30440 +0.0
BPHYT_RS30465 -1.5
BPHYT_RS30795 +0.0
BPHYT_RS31385 -0.5
BPHYT_RS31405 N.D.
BPHYT_RS31610 -0.4
BPHYT_RS31900 +0.1
BPHYT_RS33140 N.D.
BPHYT_RS33705 +0.0
BPHYT_RS34945 N.D.
BPHYT_RS34965 N.D.
BPHYT_RS35505 -0.2
BPHYT_RS35675 +0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-13-methyltetradecanoyl-[acp]→a (2E)-13-methyltetradec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
BPHYT_RS12085 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-13-methyltetradec-2-enoyl-[acp] + NADH + H+→a 13-methyltetradecanoyl-[acp] + NAD+ (EC 1.3.1.9)
BPHYT_RS01880 -0.1
BPHYT_RS12390 +0.3
BPHYT_RS20615 -0.4
BPHYT_RS30015 N.D.
BPHYT_RS33380 +0.1
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-13-methyltetradec-2-enoyl-[acp] + NADPH + H+→a 13-methyltetradecanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
BPHYT_RS32935 +0.0
Beta-ketoacyl-acyl-carrier-protein synthase II:
a 13-methyltetradecanoyl-[acp] + a malonyl-[acp] + H+→a 15-methyl-3-oxohexadecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
BPHYT_RS14415 N.D.
BPHYT_RS15130 +0.0
Oleoyl-[acyl-carrier-protein] hydrolase:
a 13-methyltetradecanoyl-[acp] + H2O→13-methyltetradecanoate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
No genes
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 15-methyl-3-oxohexadecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-15-methylhexadecanoyl-[acp] + NADP+ (EC 1.1.1.100)
BPHYT_RS01050 +0.3
BPHYT_RS04770 -0.2
BPHYT_RS05365 +0.4
BPHYT_RS05385 -0.1
BPHYT_RS06420 N.D.
BPHYT_RS07065 +0.5
BPHYT_RS08285 N.D.
BPHYT_RS08365 +0.4
BPHYT_RS08890 -0.0
BPHYT_RS10170 +0.1
BPHYT_RS10200 N.D.
BPHYT_RS10595 +0.2
BPHYT_RS10630 +0.1
BPHYT_RS10735 N.D.
BPHYT_RS11845 N.D.
BPHYT_RS13055 +2.8
BPHYT_RS14425 N.D.
BPHYT_RS15135 +0.2
BPHYT_RS15890 -0.2
BPHYT_RS16040 N.D.
BPHYT_RS16940 +0.6
BPHYT_RS20510 N.D.
BPHYT_RS20625 -0.3
BPHYT_RS20775 -0.2
BPHYT_RS21600 +0.3
BPHYT_RS22135 +2.5
BPHYT_RS25030 -0.3
BPHYT_RS25415 N.D.
BPHYT_RS25655 +0.8
BPHYT_RS26470 -1.1
BPHYT_RS28250 N.D.
BPHYT_RS30440 +0.0
BPHYT_RS30465 -1.5
BPHYT_RS30795 +0.0
BPHYT_RS31385 -0.5
BPHYT_RS31405 N.D.
BPHYT_RS31610 -0.4
BPHYT_RS31900 +0.1
BPHYT_RS33140 N.D.
BPHYT_RS33705 +0.0
BPHYT_RS34945 N.D.
BPHYT_RS34965 N.D.
BPHYT_RS35505 -0.2
BPHYT_RS35675 +0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-15-methylhexadecanoyl-[acp]→a (2E)-15-methylhexadec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
BPHYT_RS12085 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-15-methylhexadec-2-enoyl-[acp] + NADH + H+→a 15-methylhexadecanoyl-[acp] + NAD+ (EC 1.3.1.9)
BPHYT_RS01880 -0.1
BPHYT_RS12390 +0.3
BPHYT_RS20615 -0.4
BPHYT_RS30015 N.D.
BPHYT_RS33380 +0.1
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-15-methylhexadec-2-enoyl-[acp] + NADPH + H+→a 15-methylhexadecanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104)
BPHYT_RS32935 +0.0
Oleoyl-[acyl-carrier-protein] hydrolase:
a 15-methylhexadecanoyl-[acp] + H2O→15-methylhexadecanoate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information