MetaCyc Pathway: 1-butanol autotrophic biosynthesis (engineered) in Rhodopseudomonas palustris CGA009

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Reactions and Genes Sebacic (C); anaerobic
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2 H2O[in] + hν[cytosol] + 2 a plastoquinone + 4 H+[out]→2 a plastoquinol + dioxygen[in] + 4 H+[in] (EC 1.10.3.9)
No genes
a plastoquinol + 2 an oxidized plastocyanin[in] + 2 H+[out]→2 a reduced plastocyanin[in] + a plastoquinone + 4 H+[in] (EC 7.1.1.6)
No genes
a reduced plastocyanin[in] + hν[cytosol] + an oxidized ferredoxin [iron-sulfur] cluster[out]→a reduced ferredoxin [iron-sulfur] cluster[out] + an oxidized plastocyanin[in] (EC 1.97.1.12)
No genes
Ferredoxin--NADP(+) reductase:
2 a reduced ferredoxin [iron-sulfur] cluster[out] + NADP+[out] + H+[out]→NADPH[out] + 2 an oxidized ferredoxin [iron-sulfur] cluster[out] (EC 1.18.1.2)
TX73_008105 +0.1
Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating) (in reverse):
3-phospho-D-glyceroyl phosphate + NADPH + H+→D-glyceraldehyde 3-phosphate + NADP+ + phosphate (EC 1.2.1.13)
TX73_004865 N.D.
Triose-phosphate isomerase:
D-glyceraldehyde 3-phosphate→glycerone phosphate (EC 5.3.1.1)
TX73_014970 N.D.
Fructose-bisphosphate aldolase (in reverse):
glycerone phosphate + D-glyceraldehyde 3-phosphate→β-D-fructofuranose 1,6-bisphosphate (EC 4.1.2.13)
TX73_004845 +0.1
TX73_024105 N.D.
Fructose-bisphosphate aldolase (in reverse):
glycerone phosphate + D-erythrose 4-phosphate→D-sedoheptulose 1,7-bisphosphate (EC 4.1.2.13)
TX73_004845 +0.1
TX73_024105 N.D.
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
TX73_012945 +0.1
TX73_024120 N.D.
Sedoheptulose-bisphosphatase:
D-sedoheptulose 1,7-bisphosphate + H2O→D-sedoheptulose 7-phosphate + phosphate (EC 3.1.3.37)
No genes
Transketolase:
D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate→D-ribose 5-phosphate + D-xylulose 5-phosphate (EC 2.2.1.1)
TX73_004870 -0.0
TX73_024110 -0.2
Transketolase (in reverse):
β-D-fructofuranose 6-phosphate + D-glyceraldehyde 3-phosphate→D-xylulose 5-phosphate + D-erythrose 4-phosphate (EC 2.2.1.1)
TX73_004870 -0.0
TX73_024110 -0.2
Ribose-5-phosphate isomerase:
D-ribose 5-phosphate→D-ribulose 5-phosphate (EC 5.3.1.6)
TX73_010210 N.D.
Ribulose-phosphate 3-epimerase (in reverse):
D-xylulose 5-phosphate→D-ribulose 5-phosphate (EC 5.1.3.1)
TX73_019755 N.D.
Phosphoribulokinase:
D-ribulose 5-phosphate + ATP→D-ribulose-1,5-bisphosphate + ADP + H+ (EC 2.7.1.19)
TX73_024115 N.D.
Ribulose-bisphosphate carboxylase (in reverse):
D-ribulose-1,5-bisphosphate + CO2 + H2O→2 3-phospho-D-glycerate + 2 H+ (EC 4.1.1.39)
TX73_001365 -0.1
TX73_008010 +0.7
TX73_008015 -0.3
TX73_011190 +0.2
TX73_024100 -0.1
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
TX73_001765 N.D.
Phosphoglycerate kinase:
3-phospho-D-glycerate + ATP→3-phospho-D-glyceroyl phosphate + ADP (EC 2.7.2.3)
TX73_004860 N.D.
Phosphopyruvate hydratase:
2-phospho-D-glycerate→H2O + phosphoenolpyruvate (EC 4.2.1.11)
TX73_014890 N.D.
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
TX73_011750 -0.0
TX73_021735 N.D.
pyruvate + coenzyme A + NAD+→CO2 + acetyl-CoA + NADH (EC 1.2.1.104)
TX73_014840 +0.2
TX73_014850 +0.0
TX73_014855 +0.0
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
TX73_002660 +0.1
TX73_002750 -0.1
TX73_004215 -0.1
TX73_008765 +0.2
TX73_009030 +0.1
TX73_011915 +0.2
TX73_016520 -0.0
TX73_017920 +0.1
TX73_017940 -0.1
TX73_019245 -1.2
TX73_022530 +0.0
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
TX73_004220 -0.0
TX73_008775 -0.1
TX73_011910 -0.1
TX73_018155 -0.0
TX73_019255 -1.4
TX73_024670 -0.8
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
TX73_001990 -0.1
TX73_002165 -0.0
TX73_002395 +0.1
TX73_002500 +0.1
TX73_002510 +0.0
TX73_003370 +0.0
TX73_004930 +0.2
TX73_006355 +0.1
TX73_007240 +0.4
TX73_008810 -0.0
TX73_009040 -0.1
TX73_009055 +0.3
TX73_009185 +0.1
TX73_009315 -0.2
TX73_011975 +0.0
TX73_014325 +0.2
TX73_017085 -0.0
TX73_019255 -1.4
TX73_019650 -0.1
TX73_022390 -0.1
TX73_022490 +0.3
Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
crotonyl-CoA + NADH + H+→butanoyl-CoA + NAD+ (EC 1.3.1.44)
TX73_002490 +0.0
TX73_003380 +0.1
TX73_003825 -0.1
TX73_005120 -0.0
TX73_006350 +0.1
TX73_008280 +0.1
TX73_008790 +0.0
TX73_008795 +0.1
TX73_008800 +0.0
TX73_009060 +0.1
TX73_011025 +0.3
TX73_011920 +0.2
TX73_019235 -1.8
TX73_023655 -0.0
Butanal dehydrogenase (in reverse):
butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information