MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Rahnella sp. WP5

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Reactions and Genes L-Glutamine (N); ShewMM media; no shaking
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Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
EX31_RS03660 -0.6
EX31_RS03665 -0.6
EX31_RS04250 -0.0
EX31_RS14990 -0.9
EX31_RS14995 -0.6
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
EX31_RS15020 -5.7
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
EX31_RS15005 -5.8
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
EX31_RS00895 +0.1
EX31_RS08040 +0.1
EX31_RS10075 -1.4
EX31_RS10950 +0.3
EX31_RS15570 -0.0
EX31_RS16670 -0.6
EX31_RS18715 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information